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Recombinant human serum albumin and yeast ferment mixture, composition containing said mixture, preparation process and use thereof

A technology of human albumin and mixture, applied in the biological field, can solve the problems of tight supply of raw materials, complex separation process, and singleness of blood-derived products, achieve the effect of whitening appearance and acceptability, simple process, and improvement of wrinkles

Active Publication Date: 2006-12-13
NCPC NEW DRUG RES & DEV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0013] From the above patents, it can be found that part of the albumin used in cosmetics comes from the separation and purification of human blood or animal blood, and the other part comes from recombinant gene products, all of which have undergone a complicated separation process and are a single component.
Blood-derived products are still in short supply of raw materials, and there are potential threats of HIV virus, hepatitis virus and mad cow disease virus contamination in the product

Method used

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  • Recombinant human serum albumin and yeast ferment mixture, composition containing said mixture, preparation process and use thereof
  • Recombinant human serum albumin and yeast ferment mixture, composition containing said mixture, preparation process and use thereof
  • Recombinant human serum albumin and yeast ferment mixture, composition containing said mixture, preparation process and use thereof

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preparation example

[0050] The following preparation examples, examples, and experimental examples only further illustrate the present invention, and should not be construed as limiting the present invention.

[0051] Sources of main raw and auxiliary materials: recombinant human albumin fermented liquid prepared according to China Invention Patent Application No. 200510068171.9 (North China Pharmaceutical New Drug Research and Development Company); lecithin (phospholipid gmbh); glycerin (Zhangjiakou Chemical Reagent Factory); stearyl alcohol (Shanghai Huadong Normal University chemical plant, imported packaging); Lanolin (Tianjin Chemical Reagent Co., Ltd.); Cholesterol (Beijing Chemical Reagent Company); Vaseline (Shanghai Hualing Rehabilitation Equipment Factory); Chunguang Reagent Factory); Carbomer (Noyu Chemical Co., Ltd., USA); Membrane (Shanghai Xingya Purification Material Factory); the rest are commercially available.

[0052] Instruments: 79-3 constant temperature magnetic stirrer (Sha...

preparation example 1

[0055] (1) Primary desalination treatment

[0056] Pasteur's methanolic yeast containing human albumin gene was constructed by PCR technology and fermented in an 800L tank at low temperature for 8 days, and the fermentation broth was centrifuged at 10,000 rpm for 30 minutes at high speed to obtain the fermentation supernatant.

[0057] The supernatant was filtered through a 1.2um membrane to remove residual insoluble matter, and was separated into a G-25fine medium (produced by Pharmacia, Sweden) with a loading volume of 3% of the column volume.

[0058] (2) heat treatment

[0059] Collect the G-25 component (called component A) comprising rHSA, add heat stabilizer sodium caprylate, metal chelating agent EDTA-2Na and add 1% (W / V ratio) gac after carrying out 60 ℃, heat for 60 minutes, Inactivation of protease activity, heat stabilizer sodium caprylate, and metal chelating agent EDTA-2Na are added according to the Chinese invention patent application number 200510068187.X.

...

preparation example 2

[0065] Same as Preparation Example 1, except that the sample loading in the first desalination treatment in step (1) is carried out at 20% column volume, and the separation medium is G-15 (produced by Pharmacia, Sweden) for separation. The preferred 4% (W / V ratio) of active carbon add-on in step (2) heat treatment, carry out 80 ℃, heat treatment in 10 minutes, the sodium citrate of step (3) changes sodium phosphate solution into.

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Abstract

The invention discloses a blend of recombinant human blood albumin and ferment, which is prepared from Pichia pastoris containing human blood albumin gene constructed by PCR technique through fermentation culture and separating extraction processes. The process can realize large scale manufacture. The invention also discloses the use of the compositions containing the blend in skin moisture maintenance, crease-resistance and beauty treatment.

Description

technical field [0001] The invention belongs to the field of biotechnology, and further, the invention belongs to a mixture of recombinant biological products, a composition containing the mixture, its preparation process and application. Background technique [0002] Looking back at the evolution history of cosmetics: the first generation is purely physical and ordinary oil-based cosmetics; the second generation is oil-water mixing technology cosmetics; the third generation is pure natural cosmetics made of essences extracted from plants; The fourth generation is bionic cosmetics, which use biotechnology to extract from animal bodies to manufacture biological products that are similar to the natural structure of the human body and have a slight affinity. With the advancement of science and technology and the constant requirements of the beauty industry, bionic cosmetics are quickly replaced by a new generation of cosmetics. The fifth generation of pure biological cosmetics...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K8/99A61K8/64A61K8/06A61Q19/00C12P21/02
Inventor 李梅彦王会娟贾茜白玉孟雅娟任力伟沈旭东郑煜陈丽华高健仓基勇
Owner NCPC NEW DRUG RES & DEV
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