Pseudomonas RRj228 helpful for crop growth and microbe preparation containing the same

A microbial agent, Pseudomonas technology, applied in the field of environmental compatibility microbial agents, can solve problems such as crop quality degradation, soil nutrient imbalance, river eutrophication, etc., to reduce usage, increase yield, and promote plant growth Effect

Active Publication Date: 2006-12-13
韩国农村振兴厅
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Fertilizer is an important agricultural material that determines the yield of crops, but as an inferior factor for crop growth, it will lead to crop quality degradation, eutrophication of rivers, groundwater pollution, soil nutrient imbalance and reduced yield

Method used

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  • Pseudomonas RRj228 helpful for crop growth and microbe preparation containing the same
  • Pseudomonas RRj228 helpful for crop growth and microbe preparation containing the same
  • Pseudomonas RRj228 helpful for crop growth and microbe preparation containing the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Isolation of soil microorganism RRj228

[0034] The isolation method of soil microorganism RRj228 is as follows.

[0035] Such as figure 1 As shown, a rice seed germinated in a sterile medium was transferred to a 0.6% agarose medium. 0.5 g of soil sample (from St. Petersburg, Russia) was inoculated at a distance of 1-2 cm from the roots of rice, and then the medium was cultured at 28°C for 48 hours. After the roots are crushed to form a suspension, the suspension is spread on an LC medium (the medium contains 10 grams of tryptone for bacterial culture, 5 grams of yeast extract, MgSO 4 ·7H 2 O 8 grams, 1 M Tris 1 ml and distilled water 1 liter) to isolate the strain.

Embodiment 2

[0037] Identification of soil microorganism RRj228

[0038] A. Physiological and biochemical characteristics

[0039] The physiological and biochemical characteristics of the RRj228 strain are shown in Table 1 below.

[0040] The RRj228 strain is rod-shaped with a size of 2.0-3.0×0.5-0.7 microns, such as Figure 2a with 2b It is shown to have flagella. When the strain is cultured in PDB medium for 24 hours, yellowish reddish colonies will be formed. The RRj228 strain is a gram-negative bacteria that performs aerobic metabolism and has catalase and oxidase activities. When the carbon source added to the medium is arabinose, fructose, galactose, glucose, mannitol, sucrose, glycerol or xylose, the strain will produce acid, and when the carbon source added to the medium is lactose, dextrin, ramnose , Sorbitol, maltose or galactitol, the strain will produce alkali. In addition, such as image 3 As shown, the RRj228 strain of the present invention is characterized by being present outsid...

experiment Embodiment 1

[0048] Plant growth promoting effect of Pseudomonas RRj228

[0049] In order to test the plant growth promotion effect of Pseudomonas RRj228, experiments were carried out on corn seedlings. The 3 mm end of the corn root was immersed in the strain culture suspension for 1.5 hours, and the other corn roots were immersed in the sterile culture as a control. Place the corn seedlings on wet filter paper in a plastic box, then cover the end of the root at the starting line with filter paper, and incubate at 28°C for 24 hours. The length of the newly elongated roots was measured and repeated 10 times. The corn growth promoting effect of the RRj228 strain is shown in Table 2 below.

[0050] [Table 2]

[0051]

[0052] As shown in Table 2, the stock solution (4.5×10 9 cfu / ml) was diluted 1:50 and inoculated, the growth promotion level of Pseudomonas RRj228 of the present invention was reduced by 26%, and when the stock solution was diluted 1:100 and inoculated, the growth promoti...

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Abstract

The invention relates the pseudomonas RRj228, which is used to replace fertilizer to promote the plant growth. The invention also relates the secondary metabolite, environment affinity microbiological agent, which is produced by pseudomonas RRj228.

Description

Technical field [0001] The present invention relates to Pseudomonas sp. RRj228 (Pseudomonas sp. RRj228) represented by sequence No. 1 (this strain was deposited in the Korean Agricultural Culture Collection (KACC) on June 11, 2003, and the deposit number is KACC 91052; It was re-deposited in the Korea Type Culture Collection (KCTC) on May 27, 2005, and the deposit number is KCTC 10812BP). In the process of planting crops, this strain replaces chemical fertilizers by colonizing the roots of plants to promote plants Grow. The present invention also relates to environmentally compatible microbial preparations containing Pseudomonas RRj228 or secondary metabolites produced therefrom. [0002] The environmentally compatible microbial preparation according to the present invention can reduce the use of chemical fertilizers, stabilize crop yields, protect water quality and soil environment, and improve the health of the soil ecosystem by containing Pseudomonas RRj228 or secondary metabol...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08A01N63/00C12R1/38
CPCC05F11/08C12N1/20
Inventor 姜渭金全元泰朴琪都金旼兑朴昌荣V·切博塔里
Owner 韩国农村振兴厅
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