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Process for producing acrylic acid by microbiological method

A microbial method, acrylic acid technology, applied in the direction of microorganism-based methods, microorganisms, biochemical equipment and methods, etc., can solve the problems of large equipment investment, catalyst pollution, complex process, etc., and achieve low equipment requirements, high conversion rate, and technology. Simple process effect

Inactive Publication Date: 2006-12-20
SHANDONG POLYMER BIO CHEM CO LTD
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Problems solved by technology

At present, the domestic acrylic acid series products all adopt the two-step oxidation method of propylene, and foreign technology and equipment are introduced. Due to the introduction of foreign technology, it is highly dependent on foreign technology, the catalyst is seriously polluted, the investment in equipment is large, the process is complicated, and the cost is high.

Method used

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  • Process for producing acrylic acid by microbiological method

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Embodiment Construction

[0010] Referring to the accompanying drawings, this production process obtains relatively high-purity acrylic acid after acrylonitrile hydrolysis and deamination extraction. Its detailed technical plan includes as follows:

[0011] (1) Screening of enzyme-producing cell strains

[0012] The nitrile hydratase with strong acid-producing ability is screened from the strains of Corynebacterium propionici, and then the strains with high enzyme production are screened, cultivated and enlarged to obtain the cell strains used in the present invention.

[0013] (2) Preparation of enzyme-producing cells

[0014] Add an appropriate amount of seed medium (10%-50%) into the triangular flask, insert the strains (about 5%) after sterilization, and culture at 20°C-40°C in a rotary shaker at 100-600rpm for 50-120°C. Hours, bottled for later use. Add an appropriate amount of seed culture medium (10%-50%) in the seed tank, insert the above-mentioned bacterial strains for bottling after steril...

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Abstract

The invention discloses a producing method of acroleic acid through microbe method, which comprises the following steps: (1) utilizing ethylformic acid corynebacterium to cultivate nitrile hydratase; (2) adopting nitrile hydratase as catalyst to produce low-density amide acroleic acid solution; (3) proceeding acidification amino-removing reaction to produce crude acroleic acid after condensing low-density amino acroleic acid through falling-film and evaporating.

Description

1. Technical field: [0001] The invention relates to a method for producing acrylic acid, in particular to a method for producing acrylic acid by a microbial method. 2. Background technology: [0002] The production of acrylic acid in my country began in the 1960s. At that time, the equipment used the hydrolysis method of propylene, which was small in scale and few in variety. In 1984, Beijing Dongfang Chemical Factory introduced a complete set of Japanese technology and equipment, and put into operation the first large-scale acrylic acid and ester production equipment in my country. At present, domestic acrylic acid series products all adopt the two-step oxidation method of propylene, and foreign technology and equipment are introduced. Due to the introduction of foreign technology, there are strong dependence on foreign technology, serious catalyst pollution, large equipment investment, complicated process, and high cost. 3. Contents of the invention: [0003] The object...

Claims

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Application Information

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IPC IPC(8): C12P7/40C12R1/15
Inventor 夏春良吴时军刘皓周卫东孙中华付纲胡奎玲王曰鹏
Owner SHANDONG POLYMER BIO CHEM CO LTD
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