Method for obtaining molecular marker for identifying three line hybrid rice maintainer line character
A technology of molecular markers and maintainer lines, applied in the field of obtaining molecular markers of hybrid rice, can solve the problem that there is no special research on the high-retention traits of maintainer lines, and achieve the effects of wide representativeness, great practicability, and meeting the requirements of production and application
Inactive Publication Date: 2006-12-27
ZHEJIANG SCI-TECH UNIV
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AI Technical Summary
Problems solved by technology
However, there has been little dedicated research on high-maintenance traits in maintainer lines
Method used
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Experimental program
Comparison scheme
Effect test
Embodiment 2
[0029] Embodiment 2. Use the molecular markers obtained in the present invention to identify whether the "three-line" hybrid rice is an excellent variety.
[0030] 1. Select the known excellent maintainer lines "Jin 23B, KB, Zhong 9B, 064B and Digu B" as varieties to be identified. Then it is soaked, sowed, transplanted and planted according to the conventional method as a material.
[0031] 2. DNA sample preparation:
[0032] Identical to Example 1.
[0033] 3. Primer specificity detection:
[0034] PCR amplification: be equal to embodiment 1;
[0035] Gel electrophoresis: the same as in Example 1, the results showed that a 1.5 KB amplified band appeared.
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This inventino exposed a kind of molecular mark method to identify the character of three-line hybrid rice maintainer lines. The steps are: 1) collect 8-10 good maintainer lines with more than 99.9% keep rate in three-line hybrid rice, and collect another 8-10 breeding medium material with 60 - 80% maintain rate; 2) extract the gene group DNA of the above good maintainer lines and comparation; 3) se the normal AFLP molecular mark method to find the DNA amplification band that has close linkage with all good maintainer lines, which known as DNA specific bands; 4) cloning the above DNA specific band and sequencing it; 5) according to the result of sequencing, design the PCR primer which can amplificate this DNA specific band. The molecular mark obtained by this method can closely link with hign maintain character of Maintainer.
Description
technical field [0001] The invention relates to a technology for obtaining molecular markers of hybrid rice, in particular to a method for obtaining molecular markers for identifying the characteristics of maintainer lines of "three-line" hybrid rice. Background technique [0002] Ordinary "three-line" hybrid rice is composed of sterile lines, maintainer lines and restorer lines. Among them, for the reproduction of the sterile line, the interaction between the sterility gene located in the cytoplasm of the sterile line and the specific maintenance gene located in the nucleus of the maintainer line is used to maintain the male sterility traits, so the requirements for the maintainer line are very strict . However, the improvement of the maintainer line in breeding practice is very difficult and ineffective, and the time period is long and the efficiency is extremely low. As far as its reason is concerned, it seems to be due to the existence and interference of micro-effect ...
Claims
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IPC IPC(8): C12Q1/68C12N15/65
Inventor 刘小川陈集双
Owner ZHEJIANG SCI-TECH UNIV
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