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Method of implementing erythrocyte blood group antigen detection on haemocyte analysis instrument

A blood cell analyzer and blood group antigen technology, which is applied in the field of red blood cell blood group antigen detection in medicine, can solve the problems of cumbersome operation, easy misjudgment, and difficulty in judging results, and achieves a high degree of automation, high accuracy, improved accuracy and The effect of work efficiency

Inactive Publication Date: 2007-01-31
潘干华
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0021] 3. Insufficiency of current blood group antigen detection method
But it is only suitable for manual operation, and has the disadvantages of cumbersome operation, easy misjudgment, easy to be affected by condensation and false agglutination factors, making it difficult to judge the result, etc.

Method used

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  • Method of implementing erythrocyte blood group antigen detection on haemocyte analysis instrument
  • Method of implementing erythrocyte blood group antigen detection on haemocyte analysis instrument
  • Method of implementing erythrocyte blood group antigen detection on haemocyte analysis instrument

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Effect test

Embodiment 1

[0045] The present invention uses the BC-2200 semi-automatic blood cell analyzer produced by Shenzhen Mindray Company to detect the blood group antigen: because the agglutination reaction can reduce the free red blood cells and the total number of red blood cells, so the total number of red blood cells is used as a comparative analysis parameter. First determine the range of the total number of red blood cells of the instrument, and then detect the total number of red blood cells in the reaction sample and the control sample on the instrument, and calculate the change rate of the total number of red blood cells, and compare it with the predetermined range to determine whether the red blood cells exist There are blood group antigens that correspond to known blood group antibodies. The specific detection method is:

[0046] (1) Preparation of reagents and blood samples

[0047] 1. Diluent: It is a special diluent for hematology analyzer.

[0048] 2. Blood group antibody reagen...

Embodiment 2

[0065] In the present invention, the blood group antigen detection method is carried out on the BC-2800 automatic blood cell analyzer produced by Shenzhen Mindray Company: the erythrocyte agglutination particles produced by the agglutination reaction generally have a volume of about 150-250fL, so the number of erythrocytes in the 150-250fL volume segment accounts for The percentage of total red blood cell count was a comparative analysis parameter. First determine the range of the percentage of red blood cells in the 150-250fL volume segment of the instrument to the total red blood cell count, and then detect the percentage of the red blood cell count in the 150-250fL volume range of the reaction sample and the control sample on the instrument. By calculating the change rate of the red blood cell number in the 150-250fL volume segment of the two samples as a percentage of the total red blood cell number, and comparing it with the specified range, it can be judged whether there ...

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Abstract

The invention relates to a testing method for red corpuscle blood group antigen. It takes the processes of over pre-dilution, reacting, and testing, respectively testing the reacting system formed by tested red corpuscle and blood group antibody that is already known and the red corpuscle parameter of the comparing system constructed by the tested red corpuscle suspension without blood group antibody, gaining the alteration of the two system red corpuscle parameters, taking comparison analysis to the restricted range to judge whether the agglutination reaction is occurred and indirectly testing whether the tested red corpuscle has the blood type antibody corresponding to that has already been known.

Description

Technical field: [0001] The invention relates to a method for detecting red blood cell antigens in medicine, in particular to a method for detecting red blood cell antigens on a blood cell analyzer, which is mainly used in red blood cell identification. Background technique: [0002] 1. What is blood type, what is red blood cell type, what is red blood cell antigen, what is red blood cell identification, and what is red blood cell antigen detection. [0003] Blood type is one of the characteristics of human blood, which is a regular hereditary trait in the form of antigen and antibody from both parents. Initially, blood type refers to the different specific antigens present on red blood cells; later it was found that besides red blood cells, white blood cells, platelets, serum proteins, etc. all have their own unique blood types. Red blood cell blood group refers to the blood group system determined by the different specific antigenic features present on red blood cells. E...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/80G01N33/536G01N33/49
Inventor 潘干华
Owner 潘干华
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