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Enzyme linked immunoassay method for detecting content of oxygen pyrimidine in sulfanilamide-5

An enzyme-linked immunoassay and methotrexate technology, applied in the direction of measuring devices, biological testing, material inspection products, etc., can solve the problems of sample screening, cumbersome pre-treatment, and inapplicability of grassroots, etc., to achieve good market prospects and high sensitivity High, high-sensitivity effects

Inactive Publication Date: 2007-02-21
YANGZHOU UNIV
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Problems solved by technology

[0006] In particular, HPLC is often used as a statutory detection method, but because sample pretreatment is cumbersome, time-consuming, and expensive, and high-performance liquid chromatography is required, it is not suitable for grassroots and cannot be used for sample screening.

Method used

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  • Enzyme linked immunoassay method for detecting content of oxygen pyrimidine in sulfanilamide-5
  • Enzyme linked immunoassay method for detecting content of oxygen pyrimidine in sulfanilamide-5
  • Enzyme linked immunoassay method for detecting content of oxygen pyrimidine in sulfanilamide-5

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Experimental program
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Embodiment Construction

[0025] 1. Synthesis and identification of whole antigen

[0026] (1) Antigen synthesis

[0027] Weigh 112mg of sulfa-5-methoxine (SMD) and 204mg of bovine serum albumin (BSA) or 129mg of ovalbumin (OVA), dissolve in 25.5ml, pH7.2 of PB (sodium phosphate buffer ) and dioxane (ratio: 2:1), stirred on a magnetic stirrer, added dropwise 0.15ml, 25% glutaraldehyde, at room temperature, stirred for 3h, put into a dialysis bag, at 4 At ℃, dialyze with PB (pH7.0) for 6 days, change the solution twice a day, subpackage and store in freezer.

[0028] (2) Identification of synthetic antigens

[0029] Scan the SMD, the carrier protein, and their conjugates with a UV spectrophotometer at a wavelength of 200 to 400 nm to determine whether the SMD is coupled to the carrier protein.

[0030] SMD has absorption peaks at 253, 244 and 202.8nm, BSA has absorption peaks at 214 and 278nm, and conjugates form absorption peaks at 207.8, 259.4, and 265nm. It can be seen that the absorption peaks ha...

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Abstract

An enzyme linked immunodetection assay of sulfanilamide- 5-methoxymetadiazine content can change half-antigen SMD to be complete antigen for preparing specific antibody being used to determine residual SMD in foodstuff as milk, meat and egg qualitatively and quantitatively.

Description

technical field [0001] The invention relates to the technical field of safety detection of veterinary drug residues in food, in particular to the qualitative and quantitative detection of residual SMD in milk, meat, eggs and other animal foods in the market for screening and quantitative detection. Background technique [0002] Sulfonamides are widely used in veterinary clinics to treat and prevent certain diseases, but due to unreasonable use, they are likely to cause residues in animal-derived foods. Eating food containing sulfonamide drug residues can cause a series of adverse consequences, such as allergies, cancer induction, and brain damage. At present, there are several methods for the determination of sulfa-5-methoxine (ie: SMD) in animal foods such as milk, meat, eggs, etc.: [0003] 1. Microbial detection method: SMD can resist the effect of specific microorganisms, and can detect residual SMD qualitatively or semi-quantitatively. [0004] 2. Spectroscopy: UV spe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/543G01N33/52G01N33/531
Inventor 王宗元王捍东杨春花彭会建唐娜刘学忠卞建春任建新
Owner YANGZHOU UNIV
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