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Affinity chromatographic stuffing with sulfadimidine as ligand

A technology of sulfamethazine and chromatographic packing, applied in the direction of material separation, analytical materials, chemical instruments and methods, etc., can solve the problems of high cost and difficult sources

Inactive Publication Date: 2004-11-24
INST OF CHEM CHINESE ACAD OF SCI
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Problems solved by technology

They tend to be highly selective, but are costly and difficult to source

Method used

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  • Affinity chromatographic stuffing with sulfadimidine as ligand
  • Affinity chromatographic stuffing with sulfadimidine as ligand

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Embodiment Construction

[0023] 1. Select polyglycidyl methacrylate polymer microspheres (PGMA) with a monodisperse particle size as the carrier. The particle size of the microspheres is about 11 μm, which has good mechanical strength and chemical stability, and can be used in the pH range of 2 to 13. For internal use, its surface is rich in epoxy groups, with a content of 1.1mmol / g resin. Take 1.5g of PGMA microspheres, put them in a round bottom flask, add 5ml of 0.5mol / L NaOH, 5ml of ethylene glycol diglycidyl ether and 10mg of sodium borohydride, shake the reaction at room temperature on a shaker for 8 hours, suction filter and distilled water Wash and weigh 1.2g of the above-mentioned dry resin, add to the NaCO solution with 2g SMZ 3 In 30ml of buffer solution (pH10), shake and react at 50°C for 36h. The reactant was filtered, rinsed with plenty of water, and drained. Add 10ml of 1M Tris-HCl buffer solution with pH 8.5, shake and react at room temperature for 3h, filter, wash with a large amoun...

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Abstract

The present invention is one kind of affinity chromatographic stuffing with sulfadimidine as ligand specially for separating antigen globin. Sulfadimidine is one kind of widely applied sterilizing medicine, and its amino group may be utilized to bond to various carrier to prepare affinity chromatographic stuffing. The stuffing is used in specifically separating gamma antigen globin from plasma, fermented liquid, tissue homogenizing liquid and other matter containing gamma antigen globin, with purity reaching 91 %. The immobilized sulfadimidine and human IgG have interaction dissociation constant up to 3E(-6) mol / L. The new affinity chromatographic stuffing has high affinity, high safety, low cost and other features.

Description

technical field [0001] The invention belongs to a chromatographic filler and its application in the separation and purification of biological macromolecules. Specifically, it relates to an affinity chromatographic filler, that is, an affinity chromatographic filler using sulfamethazine (SMZ) as a ligand, and its application in separating and purifying gamma antibody globulin (IgG). Background technique [0002] IgG (Immunoglobulin G) plays the role of "main immunity" in humoral immunity and has important physiological functions, mainly including: 1. Neutralizing toxins and viruses; 2. Agglutination and precipitation; 3. Activating complement; 4. Cell-friendly function; 5. Immune injury, etc. The monoclonal antibody prepared by hybridoma technology belongs to homogeneous IgG and is widely used in the biochemical analysis of diseases, diagnosis and preparation of oriented drugs. However, the separation and purification of IgG has always been the bottleneck technology in its ...

Claims

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Application Information

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IPC IPC(8): B01D15/08B01J20/22
Inventor 刘国诠刘阳赵睿
Owner INST OF CHEM CHINESE ACAD OF SCI
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