Affinity chromatographic stuffing with sulfadimidine as ligand

A technology of sulfamethazine and chromatographic packing, applied in the direction of material separation, analytical materials, chemical instruments and methods, etc., can solve the problems of high cost and difficult sources
CN1548224AInactive Publication Date: 2004-11-24INST OF CHEM CHINESE ACAD OF SCI
0 Cites 15 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
INST OF CHEM CHINESE ACAD OF SCI
Publication Date
2004-11-24
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
Patent Text Reader

Abstract

The present invention is one kind of affinity chromatographic stuffing with sulfadimidine as ligand specially for separating antigen globin. Sulfadimidine is one kind of widely applied sterilizing medicine, and its amino group may be utilized to bond to various carrier to prepare affinity chromatographic stuffing. The stuffing is used in specifically separating gamma antigen globin from plasma, fermented liquid, tissue homogenizing liquid and other matter containing gamma antigen globin, with purity reaching 91 %. The immobilized sulfadimidine and human IgG have interaction dissociation constant up to 3E(-6) mol / L. The new affinity chromatographic stuffing has high affinity, high safety, low cost and other features.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to a chromatographic filler and its application in the separation and purification of biological macromolecules. Specifically, it relates to an affinity chromatographic filler, that is, an affinity chromatographic filler using sulfamethazine (SMZ) as a ligand, and its application in separating and purifying gamma antibody globulin (IgG). Background technique

[0002] IgG (Immunoglobulin G) plays the role of "main immunity" in humoral immunity and has important physiological functions, mainly including: 1. Neutralizing toxins and viruses; 2. Agglutination and precipitation; 3. Activating complement; 4. Cell-friendly function; 5. Immune injury, etc. The monoclonal antibody prepared by hybridoma technology belongs to homogeneous IgG and is widely used in the biochemical analysis of diseases, diagnosis and preparation of oriented drugs. However, the separation and purification of IgG has always been the bottleneck technology in its ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More