Method of comprehensive utilizing schisandra fruit

[0019] Example 1:

[0020] Take the south schisandra fruit powder as the raw material, weigh 20g into supercritical CO 2 Extraction unit, in CO 2 Static extraction for 5 hours at a pressure of 25MPa and an extraction temperature of 35°C; then at an extraction pressure of 25MPa, an extraction temperature of 35°C, and CO 2 Under the condition of a flow rate of 2L/min, dynamic extraction was carried out for 3 hours, and the separation temperature was 60°C. The extracts and raffinate residues of Schisandra chinensis were collected. Using schisandrae A as the reference substance, the color was developed with chromotropic acid, and the lignan content was determined by spectrophotometry. The result was that the total lignan extraction rate in the southern schisandra extract was 95.5% and the lignan content was 9.2%.

[0021] South of Schisandra supercritical CO 2 The raffinate residue is used as the raw material, the ratio of material to liquid is 1:20 (w/v, g/mL), and the boiling water is refluxed for 6 hours, extracted once, filtered, and the filtrate is concentrated under reduced pressure to 1/3 of the original volume, and the concentrate is used Trichloroacetic acid method to remove protein, add trichloroacetic acid to make the solution pH 2, ice bath for 4 hours to remove the precipitate and filter, add three times the volume of medical ethanol precipitation to the filtrate, and then use absolute ethanol, acetone, and anhydrous for precipitation Washing with ether and drying in vacuum to obtain the crude polysaccharide powder of Schisandra chinensis. The yield of crude polysaccharides in the supercritical CO2 raffinate of the south schisandra was determined to be 13.5%, and the content of polysaccharides measured by phenol-sulfuric acid method was 33.6%.

[0022] Example 2:

[0023] Using the Schisandra fruit powder as the raw material, the same method as in Example 1 was followed. Using schisandrae A as the reference substance, the color was developed by chromotropic acid, and the lignan content was determined by spectrophotometry. The result was that the total lignan extraction rate in the Schisandra chinensis extract was 93.2%, and the lignan content was 13.0%. Schisandra supercritical CO 2 The yield of crude polysaccharide in the raffinate residue was 19.6%, and the content of polysaccharide by phenol-sulfuric acid method was 28.5%.

[0024] Example 3:

[0025] Using the schisandra fruit powder as raw material, the material-to-liquid ratio is 1:10 (w/v, g/mL), 60% ethanol solution (v/v) is refluxed to extract the lignans from the schisandra. Extract twice, 2 hours each time . After filtering, the filtrate is concentrated in vacuo to obtain the extract extract, and the alcohol extraction residue is collected. Using schisandra chinensis A as the reference substance, the color was developed with chromotropic acid, and the lignan content was determined by spectrophotometry. The result was that the total lignan extraction rate in the schisandra chinensis extract was 70.8% and the lignan content was 3.6%.

[0026] The residue after extracting lignans from schisandra with 60% ethanol as raw material, with a material-to-liquid ratio of 1:20 (w/v, g/mL), and boiling water for 6 hours at the same time, extract once, filter, and concentrate the filtrate under reduced pressure. To 1/3 of the original volume, use the trichloroacetic acid method to remove the protein of the concentrated solution. Add trichloroacetic acid to make the solution pH 2. After 4 hours in an ice bath, centrifuge to remove the precipitate, filter, filter, and add three times the volume of 95% to the filtrate. Ethanol precipitation, the precipitation was washed with absolute ethanol, acetone, and anhydrous ether in turn, and dried in vacuum to obtain Schisandra polysaccharide powder. The yield of crude polysaccharides in the residue from the alcohol extraction of Schisandra chinensis was determined to be 9.25%, and the content of polysaccharides was 36.9% by phenol-sulfuric acid method.