Chinese-medicinal composition, preparation and use for treating cardiovascular diseases
A composition and a cardiovascular technology, which are applied in the application field of preparing drugs with anti-cardiomyocyte ischemia effect, can solve the problems of difficult quality control, complex chemical composition, unclear drug effect material basis, etc., and achieve simple and clear chemical composition. , the effect of easy quality control
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Embodiment 1
[0042] The preparation of embodiment one Chinese medicine composition of the present invention
[0043] Take 250 g of Danshen medicinal material, grind it, add ethyl acetate and ethanol (1:1), heat to reflux for 1 hour, extract twice, and combine the filtrates to obtain the extract. The extract is concentrated into extractum 17.2g, the extract and silica gel are mixed as a sample, and separated with a normal phase silica gel column, at first using sherwood oil and ethyl acetate (50: 1) as mobile phase to obtain eluent I, and then The mobile phase was replaced with chloroform and methanol (10:1) to obtain the eluent II, which was concentrated and dried to obtain 2.5 g of the sample. The separation of the resulting sample was continued by preparative liquid chromatography. Separation conditions for preparative chromatography: the chromatographic column is a Hanbang semi-preparative column (Lichrospher C18; 10.0mm×250mm, 5 μm), the mobile phase is water (A) and acetonitrile (B),...
Embodiment 2
[0045] The preparation of embodiment two Chinese medicine compositions of the present invention
[0046] Extract by the method of embodiment one and obtain Danshen extract and paeonol extract, 5.2 * 10 -6 gram salvia extract with 4.8 x 10 -6 The Cortex katan extract was mixed to obtain mixture A.
Embodiment 3
[0047] The preparation of embodiment three Chinese medicine compositions of the present invention
[0048] Extract by the method of embodiment one and obtain Danshen extract and paeonol extract, 4.2 * 10 -6 gram salvia extract with 5.8 x 10 -6 The kotan bark extract was mixed to obtain mixture B.
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