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Polypeptide segment capable of enhancing animal's antibody response and its application

A technology of polypeptide fragment and antibody response, applied in the fields of immunology and genetic engineering, can solve the problem of inability to induce neutralizing antibody response in animals

Inactive Publication Date: 2010-05-19
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, fusion proteins containing this peptide did not induce a sufficiently high neutralizing antibody response in animals

Method used

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  • Polypeptide segment capable of enhancing animal's antibody response and its application
  • Polypeptide segment capable of enhancing animal's antibody response and its application
  • Polypeptide segment capable of enhancing animal's antibody response and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] Example 1, the immunostimulatory protein pWRB1 expression plasmid was constructed using β-galactosidase as the carrier protein. The result is as follows:

[0015] SDS-PAGE electrophoresis detection proves that the expressed fusion protein LacZ-B1 molecular weight is consistent with expectations (see figure 1 A). Western blotting test proves that the LacZ-B1 fusion protein has strong antigenicity and can react with standard antibodies specifically (see figure 1 (B)).

[0016] Guinea pigs were immunized with the fusion protein, and T cell proliferation was detected. The results prove that the 1-33 peptide in VP2 can effectively induce animal T cell proliferation (see figure 2 ). The abscissa in the figure represents the standard antigen dilution factor X, and the ordinate represents the stimulation index SI (Note: This experiment uses the MTS / PMS method to measure the proliferation of T cells, and the stimulation index is based on the OD value of the experimental gr...

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PUM

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Abstract

The present invention belongs to the field of immunology and genetic engineering technology, and is especially one polypeptide segment capable of enhancing animal's antibody response level and its application. The polypeptide segment can stimulate genetic engineering vaccine to induce response level of neutralizing antibody, and is especially the 1-33 positions amino acid sequence of foot-and-mouth disease virus VP2 protein. The present invention adopts beta-galactosidase as carrier protein and connects the said immune stimulating polypeptide segment to the C end of the carrier protein to prepare fusion protein LacZ-B1. The fusion protein LacZ-B1 may be used as the immune stimulating adjuvant of foot-and-mouth disease genetic engineering polypeptide vaccine, and has high safety and capacity of raising animal's antibody response level.

Description

technical field [0001] The invention belongs to the technical field of immunology and genetic engineering, and specifically relates to a polypeptide fragment capable of enhancing animal antibody response and application thereof. Background technique [0002] The International Veterinary Bureau ranks foot-and-mouth disease as the first class A infectious disease. Foot-and-mouth disease is the most serious livestock infectious disease in the world today, mainly harming pigs, cattle, sheep and other cloven-hoofed animals. For many years, foot-and-mouth disease has broken out and become popular in a large scale all over the world, causing huge economic losses to animal husbandry. Vaccination is the main means of controlling the virus. Among various vaccines for preventing foot-and-mouth disease, genetically engineered vaccines have broad application prospects due to their advantages of good safety, easy preservation and stable effect. The key to the success of a genetically e...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/09A61K39/39A61K39/385A61P31/12
Inventor 郑兆鑫严维耀王加龙刘明秋陈维灶韩姬
Owner FUDAN UNIV