Recombinant L-251 protein with anti-inflammatory effect secreted by Japanese lamprey oral gland

A technique for oral glands and lampreys, which is applied in the field of recombinant Japanese lamprey oral glands to secrete L-251 protein with anti-inflammatory effect, which can solve problems such as tissue and cell damage.

Inactive Publication Date: 2010-12-29
LIAONING NORMAL UNIVERSITY
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] On the one hand, the inflammatory response can remove foreign antigens or pathogenic microorganisms, but on the other hand, it can cause tissue and cell damage

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Recombinant L-251 protein with anti-inflammatory effect secreted by Japanese lamprey oral gland
  • Recombinant L-251 protein with anti-inflammatory effect secreted by Japanese lamprey oral gland
  • Recombinant L-251 protein with anti-inflammatory effect secreted by Japanese lamprey oral gland

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0050] 1. Extraction of total RNA: Trizol reagent from GIBCO BRL was used. details as follows:

[0051] (1) Take the Japanese lamprey oral gland and place it in liquid nitrogen to grind rapidly;

[0052] (2) Weigh 0.2 g glandular tissue and secretion, add 1 ml TRIZOL reagent to prepare homogenate, and incubate at 4°C for 5 min;

[0053] (3) Add 0.2ml chloroform, close the cap tightly, shake vigorously for 15 seconds, and then place on ice for 5 minutes;

[0054] (4) 4°C, 12000g, centrifuge for 15 minutes;

[0055] (5) Transfer the upper aqueous phase to another centrifuge tube, add 0.5ml isopropanol, and incubate on ice for 10 min;

[0056] (6) 4°C, 12000g, centrifuge for 15 minutes;

[0057] (7) Discard the supernatant, add 1ml of 75% ethanol to the precipitate (containing RNA) to wash, and vortex to mix;

[0058] (8) Centrifuge at 10,000 g for 5 min at 4°C to obtain RNA pellets;

[0059] (9) After air drying, dissolve with appropriate amount of TE or RNase-free water f...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
molecular weightaaaaaaaaaa
molecular weightaaaaaaaaaa
Login to view more

Abstract

The invention discloses a cDNA sequence and cloning of L-251 protein excreted by Japanese lamprey oral gland and amino acid sequence corresponding to cDNA and expression in the engineering bacteria as well as connecting protein of CD11a and CD11b beta2 integrated agent (CR3 receptor), which inhibits neutrophil leucocyte from transmitting from endothelium cell to inflammation point.

Description

technical field [0001] Identification of neutrophil inhibitory activity of recombinant Japanese lamprey oral gland secretion PR-1 protein L-251 belongs to the field of biotechnology, involving the sequence and cloning of the cDNA of the Japanese lamprey oral gland secretion L-251 protein cDNA, and the amino acids corresponding to the cDNA The sequence and its expression in Escherichia coli or Pichia pastoris, and L-251 protein as the binding protein of CD11a and CD11bβ2 integrin can block the CD11b / CD18 and CD11a / CD18 integrin specifically expressed by neutrophils (CR3 receptor body), thereby inhibiting the migration of neutrophils across endothelial cells to sites of inflammation. It is suggested that the lamprey, a parasitic organism, escapes the host's inflammatory response. In addition to viruses, worms and other species, this is a new species that has escaped the host's immune monitoring mechanism, and it is also a potential treatment and prevention caused by inflammatory...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/63C12N15/12C07K14/435A61K38/17A61P31/00C12N1/21
Inventor 李庆伟吴毓白洁王继红
Owner LIAONING NORMAL UNIVERSITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap