Plant activator
a plant activator and plant technology, applied in the field of plant activators, can solve the problems of requiring a large amount of energy, and achieve the effect of promoting the formation of flower buds
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[0130] The present invention will next be described in detail by way of Examples, which should not be construed as limiting the invention thereto.
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Production of Specific Ketol Fatty Acid (I)
[0131] Specific ketol fatty acid (I) [9-hydroxy-10-oxo-12(Z),15(Z)-octadecadienoic acid] was produced by means of an enzyme method as follows.
[0132] 1. Preparation of Rice-Germ-Derived Lipoxygenase
[0133] Rice germ (350 g) was washed with petroleum ether, defatted, and then dried. The resultant rice germ (250 g) was suspended in a 0.1 M acetate buffer solution (pH 4.5) (1.25 L), and the resultant suspension was homogenized.
[0134] Subsequently, the resultant homogenized extract was subjected to centrifugation at 16,000 rpm for 15 minutes, to thereby yield a supernatant (0.8 L). Ammonium sulfate (140.8 g) (30% saturation) was added to the supernatant, and the resultant mixture was allowed to stand at 4° C. overnight. Thereafter, the mixture was subjected to centrifugation at 9,500 rpm for 30 minutes, to thereby yield a supernatant (0.85 L). Ammonium sulfate (232 g) (70% saturation) was added to the supernatant, after which the resultant mi...
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