Unlock instant, AI-driven research and patent intelligence for your innovation.

Novel RGS-containing molecules and uses thereof

a technology of rgs and molecules, applied in the field of rgs (regulators of gprotein signaling) nucleic acids and proteins, can solve the problems of limiting the duration of g/sub>signaling, reducing the half-life of active gtp-bound state, etc., and achieves the effect of modulating rgs activity and rgs protein expression

Inactive Publication Date: 2005-07-14
MILLENNIUM PHARMA INC
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0017] In yet another aspect, the invention provides a method for modulating RGS activity comprising contacting a cell with an agent that modulates (inhibits or stimulates) RGS activity or expression such that RGS activity or expression in the cell is modulated. In one embodiment, the agent is an antibody that specifically binds to RGS protein. In another embodiment, the agent modulates expression of RGS protein by modulating transcription of an RGS gene, splicing of an RGS mRNA, or translation of an RGS mRNA. In yet another embodiment, the agent is a nucleic acid molecu

Problems solved by technology

RGS proteins appear to enhance the endogenous GTPase activity of G-proteins, thus decreasing the half-life of the active GTP-bound state and limiting the duration of Gαi signaling.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Novel RGS-containing molecules and uses thereof
  • Novel RGS-containing molecules and uses thereof
  • Novel RGS-containing molecules and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0025] The present invention provides isolated nucleic acid molecules comprising a nucleotide sequence encoding the RGS polypeptides whose amino acid sequence is given in SEQ ID NO:2 or 4 respectively, or a variant or fragment of the polypeptide. Nucleotide sequences encoding the RGS proteins of the invention are set forth in SEQ ID NOS:1 and 3.

[0026] The present invention relates to methods and compositions for the modulation, diagnosis, and treatment of immune and respiratory disorders, especially RGS related disorders. Such immune disorders include, but are not limited to, chronic inflammatory diseases and disorders, such as Crohn's disease, reactive arthritis, including Lyme disease, insulin-dependent diabetes, organ-specific autoimmunity, including multiple sclerosis, rheumatoid arthritis, inflammatory bowel disease, Hashimoto's thyroiditis and Grave's disease, contact dermatitis, psoriasis, graft rejection, graft versus host disease, sarcoidosis, atopic conditions, such as as...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Temperatureaaaaaaaaaa
Temperatureaaaaaaaaaa
Fractionaaaaaaaaaa
Login to View More

Abstract

Novel RGS polypeptides, proteins, and nucleic acid molecules are disclosed. In addition to isolated, full-length RGS proteins, the invention further provides isolated RGS fusion proteins, antigenic peptides, and anti-RGS antibodies. The invention also provides RGS nucleic acid molecules, recombinant expression vectors containing a nucleic acid molecule of the invention, host cells into which the expression vectors have been introduced, and nonhuman transgenic animals in which an RGS gene has been introduced or disrupted. Diagnostic, screening, and therapeutic methods utilizing compositions of the invention are also provided.

Description

CROSS REFERENCE TO RELATED APPLICATION [0001] This application is a divisional of U.S. application Ser. No. 09 / 244,314, filed Feb. 4, 1999, herein incorporated by reference in its entirety.FIELD OF THE INVENTION [0002] The invention relates to novel RGS (regulators of G-protein signaling) nucleic acids and proteins. Also provided are vectors, host cells, and recombinant methods for making and using the novel molecules. BACKGROUND OF THE INVENTION [0003] Regulators of G-protein signaling (RGS) accelerate guanosine triphosphate (GTP) hydrolysis by Gi, but not by Gs class α-subunits (Popov et al. (1997) Proc. Natl. Acad. Sci. USA 94: 7216-20). RGS proteins were first identified in genetic screens in fungi and nematodes as negative regulators of G-protein signaling (Dolhman et al. (1995) Mol. Cell. Biol. 15: 3635-43). RGS proteins have been shown to-function as GTPase-activating proteins. It has additionally been proposed that RGS proteins may act as effector antagonists by occluding th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/53A61K38/00C07K14/47C07K16/18C12N5/10C12N15/09C12P21/02C12Q1/02C12Q1/68G01N33/566
CPCA61K38/00C07K2319/00C07K14/4702Y02A50/30
Inventor HODGE, MARTINYOWE, DAVID
Owner MILLENNIUM PHARMA INC