Method and apparatus for depositing samples on a target surface

a target surface and sample technology, applied in the direction of component separation, particle separator tubes, liquid/fluent solid measurement, etc., can solve the problems of difficult coupling to maldi, low abundance of peptides that are not easily detected, and the effect of rapid and flexible manner

Inactive Publication Date: 2007-02-01
UNIVERSITY OF MANITOBA
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  • Abstract
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  • Claims
  • Application Information

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Benefits of technology

[0007] By using the target surface as a sealing member, the vacuum activated drop deposition device of the present invention provides a simplified set up for separating and depositing sample drops in rapid and flexible manner.

Problems solved by technology

Although matrix assisted laser desorption / ionization (MALDI) is very effective for screening high abundance proteins in complex samples, lower abundance peptides often remain undetected.
Suppression effects are a common problem arising from the presence of multiple analytes competing for protons during the ionization process.
However, efficient coupling to MALDI have been more difficult.
However, their apparatus comprises a cumbersome vacuum chamber box in which the MALDI target and the separation device are inserted.
Drop deposition is made difficult by the limited number of degrees of freedom for displacing the tip of the separation device relative to the target.

Method used

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  • Method and apparatus for depositing samples on a target surface
  • Method and apparatus for depositing samples on a target surface
  • Method and apparatus for depositing samples on a target surface

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Embodiment Construction

[0017] A vacuum activated sample depositing / separating device is provided for deposition of samples on a target surface such as a MALDI target surface for mass spectral analysis of the sample.

[0018] An embodiment of the sample depositing device of the present invention is shown in FIG. 1. The device comprises a housing 10 which in turn comprises a vacuum chamber 11, a sealable opening 12 communicating with the vacuum chamber, a sample channel 14 comprising a sample inlet 16 connected to a sample source and a sample outlet 18 located inside the vacuum chamber.

[0019] The sample can be circulated directly in the sample channel 14 but, alternatively, it is also possible to insert a sample duct 15 within the sample channel or directly within the vacuum chamber. The sample duct may be adapted to help control the flow of the sample depending for example on the viscosity of the sample. Thus the size, shape and material of the duct may be chosen as function of the sample to be deposited. F...

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Abstract

There is provided a sample deposition device for depositing samples on a target surface such as MALDI target surfaces for use in mass spectrometry analysis. The device comprises a vacuum chamber, a sealable opening communicating with the vacuum chamber, a sample inlet and a sample outlet. The sample outlet is located in the vacuum chamber which is sealed by contact with the target surface. The sample is drawn through the sample inlet and deposited on the surface by the action of the vacuum.

Description

CROSS-REFERENCE TO RELATED APPLICATION [0001] This patent application claims priority on U.S. provisional application No. 60 / 694,685 entitled “METHOD AND APPARATUS FOR DEPOSITING SAMPLES ON A TARGET SURFACE” filed on Jun. 29, 2005.FIELD OF THE INVENTION [0002] The invention relates to the field of macromolecules separating device and more specifically to the field of macromolecules separating device for mass spectrometry analysis. BACKGROUND OF THE INVENTION [0003] Mass spectrometry has become a major analytical tool in proteomic and biological research in general. Most protein identification strategies involving MS analyze proteolytic peptides (e.g. tryptic digests for mass fingerprinting in combination with tandem MS to confirm amino acid sequence and the presence of various posttranslational modifications. In most proteome studies, proteins are separated on electrophoretic gels and in-gel digestion extracts are subjected to MS analysis. Although matrix assisted laser desorption / i...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): G01F23/00
CPCG01N1/2208G01N1/2813H01J49/04G01N2030/8411G01N30/7233H01J49/0418
Inventor CHEN, VINCENT C.PERREAULT, HELENE
Owner UNIVERSITY OF MANITOBA
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