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Antigen of hybrid M protein and carrier for group a streptococcal vaccine

a streptococcal vaccine and hybrid m protein technology, applied in the field of recombinant vaccines, can solve the problems of generally non-immunogenic or inadequate immunogenic antigens, and achieve the effect of optimally raising the desired antigen, optimizing the exposure of the epitope, and promoting the conformation of the fragment of m protein

Inactive Publication Date: 2007-03-08
UNIV OF TENNESSEE RES FOUND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The antigens are generally non-immunogenic (or not adequately immunogenic) because of their molecular size or for other reasons.

Method used

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  • Antigen of hybrid M protein and carrier for group a streptococcal vaccine
  • Antigen of hybrid M protein and carrier for group a streptococcal vaccine
  • Antigen of hybrid M protein and carrier for group a streptococcal vaccine

Examples

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example i

[0091] Purification of LT-B-M24 hybrid protein. The recombinant LT-B-M24 protein was purified from cell extracts of JM105 (harboring pEC.LT-B-M24) grown overnight in one liter of L-broth supplemented with 75 μg / ml ampicillin, 25 μg / ml streptomycin and 1 mM isopropylthiogalactoside (IPTG, Bethesda Research Laboratories, Inc., Bethesda, Md.).—The cells were pelleted at 7,000×g and resuspended in 50 ml 100 mM carbonate buffer, pH 11, containing 100 μg / ml lysozyme, 1 mM ethylenediaminetetraacetic acid (EDTA, Sigma Chemical Co., St. Louis, Mo.) and 100 μg / ml phenylmethylsulfonylfluoride (PMSF, Sigma Chemical Co.) and incubated at 37° C. for 30 minutes. The cells were centrifuged at 7,000×g and the supernatant was dialyzed against distilled water and lyophilized. Purification was performed by loading 50 mg of hybrid protein extract onto a preparative polyacrylamide gel electrophoresis unit (Prep Cell, Model 491, Bio Rad., Inc.) using a 37 mm column and a 9 cm 11% polyacrylamide gel. Six m...

example 2

[0092] Immunization of rabbits. Rabbits were immunized intracutaneously with 300 μg LT-B-M24 protein emulsified in complete Freund's adjuvant. A booster injection of the same dose in saline was given four weeks later. Blood was obtained prior to the first injection and at two-week intervals thereafter for eight weeks.

example 3

[0093] Assay for M protein antibodies. Rabbit antisera were assayed for the presence of M protein antibodies by ELISA using LT-B, pep M24 or SM24 (1-29)C as solid phase antigens, as previously described. Opsonic antibodies against type 24 streptococci were assayed by in vitro opsonophagocytosis tests. Briefly, 0.1 ml of test serum was added to 50 μl of a standard suspension of streptococci. 0.4 ml heparinized, non-immune normal human blood was added and the mixture was rotated end-over-end for 30 minutes. The presence of opsonic antibodies was estimated by counting the percentage of neutrophils with associated streptococci (percent opsonization) on stained smears. Indirect bactericidal assays were performed using the same mixture as described above except that fewer streptococci were added. The tubes were rotated for three hours and pour plates were made using 0.1 ml of the test mixture in 5% sheep blood agar. CFU of streptococci surviving were counted after incubating overnight at ...

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Abstract

Recombinant hybrid streptococcal M protein antigens are provided which elicit protective antibodies against Group A streptococci and prevent rheumatic fever. Recombinant hybrid genes that encode the antigen are provided. Vaccine compositions and methods of administering the compositions are provided to elicit immunity against Group A streptococci.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS [0001] This application is a continuation of U.S. patent application Ser. No. 10 / 141,627, filed May 7, 2002, now allowed; which is a continuation of U.S. patent application Ser. No. 08 / 914,479, filed Aug. 19, 1997 (U.S. Pat. No. 6,419,932); which is a continuation of U.S. patent application Ser. No. 08 / 409,270, filed Mar. 23, 1995, now abandoned; which is a continuation of U.S. patent application Ser. No. 07 / 945,860, filed Sep. 16, 1992, now abandoned. These applications are incorporated herein by reference in their entireties.FIELD OF INVENTION [0002] The present invention relates broadly to the field of recombinant vaccines. The vaccines are directed to preventing Group A streptococcal infections, which may otherwise result in rheumatic fever. BACKGROUND OF THE INVENTION [0003] Acute rheumatic fever (ARF) is the major cause of heart disease in children around the world. The disease is rampant in developing countries where prevalence rates of...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): A61K39/09C07K14/315A61K39/00C07K14/245
CPCA61K39/00A61K39/092C07K14/245C07K14/315Y10S530/807C07K2319/40C07K2319/55Y10S530/806Y10S530/825C07K2319/00
Inventor DALE, JAMES B.
Owner UNIV OF TENNESSEE RES FOUND
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