Adenoviral expression vectors

a technology of adenovirus and vector, which is applied in the direction of dsdna viruses, drug compositions, genetic material ingredients, etc., can solve the problems of increasing transgene expression levels, and achieve the effects of reducing the expression of viral proteins, reducing the occurrence of replication competent, and increasing the expression of heterologous sequences

Inactive Publication Date: 2007-10-25
CANJI
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Benefits of technology

[0005] The present invention provides a recombinant adenovirus vector characterized by the partial or total deletion of adenoviral E2B function and having an expression cassette containing a heterologous sequence encoding a protein of interest inserted into the E1 region. Such vectors are designed to have a reduction in the occurrence of replication competent adenovirus c

Problems solved by technology

Such substitutions may result in incr

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  • Adenoviral expression vectors
  • Adenoviral expression vectors
  • Adenoviral expression vectors

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Embodiment Construction

[0020] The present invention provides, inter alia, recombinant adenoviruses characterized by the partial or total deletion of the adenoviral E2B gene and having an expression cassette capable of encoding a protein of interest inserted into the E1 region. The subject vectors will find use in therapeutic applications, in which the vectors are employed to express a therapeutic nucleic acid, e.g. gene, into the genome of a target cell, i.e. gene therapy applications. The subject vectors may be used to deliver a wide variety of therapeutic nucleic acids. Therapeutic nucleic acids of interest include genes that replace defective genes in the target host cell, such as those responsible for genetic defect based diseased conditions; genes which have therapeutic utility in the treatment of cancer, autommune and / or infectious diseases and the like.

[0021] In accordance with the present invention there may be employed conventional molecular biology, microbiology, and recombinant DNA techniques ...

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Abstract

The present invention provides a recombinant adenovirus vector characterized by the partial or total deletion of adenoviral E2B function and having an expression cassette containing a heterologous sequence encoding a protein of interest inserted into the E1 region. Such vectors are designed to reduce or eliminate the occurrence of replication competent adenovirus contamination. Additionally, the expression cassette of the vector may contain one or more regulatory elements capable of increasing the expression of the heterologous sequence and/or reducing the expression of viral proteins. Such a reduction in expression of viral proteins reduces the cytotoxicty and immunogenicity of the adenovirus vectors when administered in vivo. Transformed production host cells and a method of producing recombinant proteins and gene therapy also are included within the scope of this invention.

Description

REFERENCE TO CROSS RELATED APPLICATIONS [0001] This application claims the benefit of priority under 35 USC 119(e) of provisional patent application U.S. Ser. No. 60 / 750,012 filed Dec. 12, 2005, the disclosure of which is hereby incorporated by reference in its entirety.1. FIELD OF THE INVENTION [0002] The present invention provides a recombinant adenovirus vector characterized by the partial or total deletion of adenoviral E2B function and having an expression cassette containing a heterologous sequence encoding a protein of interest inserted into the E1 region. Such vectors are designed to reduce or eliminate the occurrence of replication competent adenovirus contamination. Additionally, the expression cassette of the vector may contain one or more regulatory elements capable of increasing the expression of the heterologous sequence and / or reducing the expression of viral proteins. Such a reduction in expression of viral proteins reduces the cytotoxicty and immunogenicity of the a...

Claims

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Application Information

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IPC IPC(8): A61K31/70C12N15/00
CPCA61K48/00C12N7/00C12N15/86C12N2830/48C12N2830/40C12N2830/42C12N2710/10343A61P35/00
Inventor MAYALL, TIMOTHY P.
Owner CANJI
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