Acoustically mediated fluid transfer methods and uses thereof
a fluid transfer and acoustic technology, applied in the field of noncontact fluid transfer methods and apparatus, can solve the problems of increasing the likelihood of contamination, reducing the efficiency of existing fluid transfer methods, so as to achieve convenient automation, improve the effect of intrinsic reliability and high viscosity
Inactive Publication Date: 2008-05-01
EDC BIOSYST
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Benefits of technology
This method significantly reduces contamination risks, enhances reliability, and allows for the creation of high-density arrays, supporting high-throughput operations with precise and efficient fluid transfer, compatible with a wide range of liquid compounds.
Problems solved by technology
Such contact methods dramatically increase the likelihood of contamination.
Contamination of the strand with an erroneously placed nucleotide renders the oligonucleotide useless.
Furthermore, existing fluid transfer methods are limited, and do not conveniently and reliably produce the high efficiency, high-density arrays.
Method used
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Embodiment Construction
[0022] In accordance with the present invention, there is provided an apparatus useful for non-contact fluid transfer. With reference to FIG. 1 there is schematically presented one embodiment of an apparatus of the present invention. The figure depicts a non-contact fluid transfer apparatus 5 having at least one acoustic liquid deposition emitter 60 in electrical communication with a computer 95. During operation the acoustic liquid deposition emitter 60 generates an acoustic wave or beam 10 that can be propagated through an optional wave channel 70. The acoustic wave can be focused by tens 75 prior to propagating through coupling fluid 20 to optimize the energy of the acoustic wave or beam 10 upon the liquid / air interface of source fluid 40. The acoustic wave 10 is propagated through a coupling medium 20 after which the wave is transmitted through source fluid containment structure 30 where the wave comes to focus at or near the surface of a pool of source fluid 40 thereby causing ...
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Abstract
Acoustic waves are used to transfer small amounts of fluid in a non-contact manner. Acoustic waves are propagated through a pool of a source fluid in such a manner that causes the ejection of a single micro-droplet from the surface of the pool. The droplet is ejected towards a target with sufficient force to provide for contact of the droplet with the target. Because the fluid is not contacted by any fluid transfer device such as a pipette, the opportunities for contamination are minimized. Methods may be employed to transfer fluids from an array of source sites to an array of target sites, thereby enabling the precise automation of a wide variety of procedures including screening and synthesis procedures commonly used in biotechnology.
Description
CROSS-REFERENCE TO RELATED APPLICATIONS [0001] This application is a continuation of U.S. patent application Ser. No. 09 / 735,709, filed on Dec. 12, 2000, the contents of which is incorporated herein by reference in its entirety.FIELD OF THE INVENTION [0002] The present invention relates to non-contact fluid transfer methods, apparatus and uses thereof. BACKGROUND [0003] Many methods for the precision transfer and handling of fluids are known and used in a variety of commercial and industrial applications. The presently burgeoning industries of biotechnology and biopharmaceuticals are particularly relevant examples of industries requiring ultra-pure fluid handling and transfer techniques. Not only is purity a concern, current biotechnological screening and manufacturing methods also require high throughput to efficiently conduct screening of compound libraries, synthesis of screening components, and the like. [0004] Current fluid transfer methods require contacting the fluid with a t...
Claims
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IPC IPC(8): C40B40/00C40B50/08G10K15/00C40B40/10B01L3/02C40B60/14G01N21/11
CPCB01J19/0046Y10T436/2575B01J2219/00378B01J2219/00527B01J2219/00585B01J2219/00596B01J2219/00605B01J2219/00612B01J2219/00637B01J2219/00641B01J2219/00659B01J2219/00725B01L3/0268B01L2300/0819B01L2400/0433B01L2400/0436B05B17/0615C40B30/04C40B40/02C40B40/04C40B40/08C40B40/10C40B50/00C40B60/14F04F7/00G01N21/11B01J2219/00362Y10T137/206Y10T137/2196
Inventor WILLIAMS, ROGER O.SINGH, TARLOCHANMANSOUR, N. NICOLASLEE, LAWRENCE JR.FORBUSH, MICHAEL J.
Owner EDC BIOSYST