Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Selective Ablation of Diploid Embryos

a technology of selective ablation and embryos, applied in the field of selective ablation of diploid embryos, can solve the problems of inefficient use of labor and time, and achieve the effect of preventing the growth of diploid embryos

Inactive Publication Date: 2008-08-07
PIONEER HI BRED INT INC +1
View PDF10 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0004]Methods for identifying haploids by preventing the growth of diploid embryos are provided. Methods for identifying haploid plants, seeds, embryos, and plant cells are provided. Methods for producing haploid inducer lines and the haploid inducer lines are provided.

Problems solved by technology

This is an inefficient use of labor and time.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0093]Seeds from haploid inducer lines, such as Stock 6, RWS, KEMS, KMS or ZMS, are planted and self or sib pollinated at flowering. The ears are surface sterilized in 30% Clorox bleach plus 0.5% Micro detergent for 20 minutes, and rinsed two times with sterile water.

[0094]For Agrobacterium-mediated transformation of maize the method of Zhao is employed essentially as described in U.S. Pat. No. 5,981,840, the contents of which are hereby incorporated by reference. Embryos (generally about 7-14 days after pollination) are isolated from a maize plant and the embryos are contacted with a suspension of Agrobacterium, where the bacteria are capable of transferring the nucleotide sequences of interest to at least one cell of at least one of the embryos. The Agrobacterium comprises the following expression cassette.

Construct C:

[0095]ZM-lec1 promoter:UB1ZMintron:barstar:pinII 3′terminator—ZM-PG47 promoter:ZM-BT1 transit peptide:ZM-alpha-amylase1:ZM-IN2-1 3′ terminator—UB1ZM promoter:5′UTR i...

example 2

Production of Haploid Embryos

[0105]After an ablation haploid inducer line is produced as indicated in Example 1. The inducer line can be used to produce haploid embryos. This can be achieved through the growing of F1 corn seed. For example a corn breeder wanting to produce a superior inbred with the best characteristics from elite inbred line A and elite inbred line B crosses the two inbreds to form F1 hybrid seed. This F1 seed is grown along with the ablation inducer line. The timing of the planting is such that the pollen of the ablation inducer line is ready at the silking time of the F1 hybrid seed. The silks of the F1 plants are pollinated with the ablation inducer line pollen. This can be achieved by planting alternate rows of the F1 and the inducer line, and various combinations thereof with the F1, or female, plants being detasseled before pollination. The crosses can also be done by shoot bagging and controlling pollination by hand pollinating.

[0106]After the seed has matur...

example 3

Transformation and Regeneration of Transgenic Inducer Maize Plants

[0107]Hi-II maize seeds are planted. The ears at 9-12 days after pollination are harvested surface sterilized in 30% Clorox bleach plus 0.5% Micro detergent for 20 minutes, and rinsed two times with sterile water. The embryos are isolated from ears using a scalpel. Embryos are contacted with a suspension of Agrobacterium, where the bacteria are capable of transferring the nucleotide sequences of interest to at least one cell of at least one of the embryos. In this example the embryos are co-transformed with 2 Agrobacteria.

[0108]One Agrobacterium comprises the following expression cassette.

Construct E:

[0109]ZM-lec1 promoter:UB1ZMintron:barstar:pinII 3′terminator—ZM-PG47 promoter:ZM-BT1 transit peptide:ZM-alpha-amylase1:ZM-IN2-1 3′ terminator—UB1ZM promoter:5′UTR intron:GAT:PINII 3′ terminator.

[0110]The second Agrobacterium comprises the following expression cassette.

Construct F:

[0111]ZM-lec1 promoter:Potato LS intron:b...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
timeaaaaaaaaaa
timeaaaaaaaaaa
timeaaaaaaaaaa
Login to View More

Abstract

Methods of selecting haploid embryos are disclosed. Methods of producing haploid embryos and non-viable diploid embryos on a plant are provided. Methods for selecting haploid embryos produced from haploid inducer maize lines are provided. Methods for producing improved maize haploid inducer lines are disclosed. Maize haploid inducer lines comprising transgenes causing ablated or abnormal diploid embryos are disclosed.

Description

CROSS REFERENCE TO RELATED APPLICATIONS[0001]This application claims the benefit of, and hereby incorporates by reference, provisional application 60 / 887,828 filed Feb. 2, 2007.THE INVENTION[0002]The present invention relates to the field of plant breeding and plant biotechnology.BACKGROUND OF THE INVENTION[0003]Homozygous plants are basic for product development and commercialization of plants. To obtain homozygous plants requires several generations of self-pollination and segregation analysis. This is an inefficient use of labor and time. It would therefore be useful to develop a method to reduce hand pollination steps normally required to obtain a homozygous plant and reduce the amount of time required to obtain a homozygous population of plants. One way to obtain homozygous plants without the need to self-pollinate multiple generations is to produce haploids and then double the chromosomes to form doubled haploids. A process to assist in the selection of haploid embryos and eli...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(United States)
IPC IPC(8): A01H1/02C12N15/29A01H5/00
CPCA01H1/08C12N15/8287C12N15/8263C12N15/8217
Inventor WILLIAMS, MARK E.GORDON-KAMM, WILLIAM J.
Owner PIONEER HI BRED INT INC
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com