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Regulator for flowering time, transgenic plant transformed with the same, and method for regulating flowering time

a transgenic plant and flowering time technology, applied in the direction of plant/algae/fungi/lichens ingredients, peptides, dna/rna fragmentation, etc., can solve problems such as inaction, and achieve the effect of accelerating flowering time and delayed flowering tim

Inactive Publication Date: 2009-05-14
POHANG IRON & STEEL CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0027]Many flowering regulators of plants have been known, most of which are flowering-time activators, but only few of them are flowering inhibitors. Therefore, it is very valuable to find flowering inhibitors such as OsMADS56 and OsCOL4. In activating flowering, the flowering inhibitor suppressed mutants can be more stably inherited than the flowering activator overexpressed mutants. Further, the OsCOL4 suppressed mutants can be obtained by ways other than transformation, such as mutation by an endogenous transposon of rice, Tos17, and thus, problems of genetically modified organisms (GMO) can be avoided.
[0029]The flowering regulators of the present invention may act independently from each other. Further, each of the regulators may more effectively control flowering-time by acting in association with another regulator with a similar or opposed regulation activity to create offset or synergy of the flowering regulating action.
[0043]The transgenic plant, part thereof, or plant cell of the present invention may be transformed with a DNA construct containing the OsMADS14 gene having the C domain coding region deleted, to produce a C domain deleted partial OsMADS14 protein, whereby flowering-time is delayed under long-day conditions and accelerated under short-day conditions.
[0053]The flowering-time regulating method of the present invention may comprise the step of inducing overexpression of a C domain deleted partial OsMADS14 protein by deletion of the 3′-terminal region containing the C domain coding sequence of the OsMADS14 gene, to delay flowering-time under long-day conditions, and to accelerate the flowering-time under short-day conditions.

Problems solved by technology

However, the CO does not act as a flowering inhibitor under short-day conditions (Putterill et al., 1995).

Method used

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  • Regulator for flowering time, transgenic plant transformed with the same, and method for regulating flowering time
  • Regulator for flowering time, transgenic plant transformed with the same, and method for regulating flowering time
  • Regulator for flowering time, transgenic plant transformed with the same, and method for regulating flowering time

Examples

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example 1

Selection 1 of Flowering-Time Mutants in T-DNA-Tagging Lines

[0089]Rice cells (Oryza sativa var. japonica cv. Dongjin induced calluses) were treated by using Ti plasmid binary vector pGA2144 (Jeon et al., The Plant Journal (2000) 22(6), 561-570), to construct a T-DNA inserted T1 (first generation) mutant lines. The treatment using Ti plasmid binary vector pGA2144 was performed according to “Jeon et al. 2000b”. That is, the rice cells were co-cultivated with agrobacteria, to transport T-DNA into the rice cells, and the T-DNA transported cells were selected by using an antibiotic and re-differentiated. 2933 T1 mutant lines obtained were developed in the field, to produce T2 (second generation) transgenic plants, wherein mutant lines exhibiting alteration in flowering-time were selected. Twenty-five (25) lines exhibiting alteration in flowering-time by at least 2 weeks compared with the flowering-time of the wild-type line were observed, wherein 16 lines exhibited early-flowering phenot...

example 2

Isolation of the OsMADS50 Gene

[0099]The nucleotide sequence of the above OsMADS50 gene is shown in SEQ ID NO: 1. The nucleotide sequence of the OsMADS50 gene is also registered in NCBI database under Accession No. AB003328. However, only the expression profiles in various organs is known, whereas its function is yet unknown (Shinozuka et al., 1999). Herein, the present inventors designed two specific primer pairs, isolated this gene through PCR using the primer pairs, and named the gene OsMADS50. The first PCR was performed for the gene using the primer pair having the nucleotide sequences of SEQ ID NO: 19 (F1: forward at 5′ UTR: 5′-atcaagcttt acggccaaac cctacagc-3′) and SEQ ID NO: 20 (R1: reverse primer at 3′ UTR: 5′-ttgggtaccg atgggtagtg gagtctgc-3′), and then, the second PCR was performed using the PCR amplified product as a template and using a primer pair correspondiing to the nucleotide sequences of SEQ ID NO: 21 (5′-atcaagcttg ttggttcatc ggcgatcg-3′) and SEQ ID NO: 22 (5′-ttg...

example 3

Analysis of OsMADS50 RNA Interference (RNAi) Plants

[0100]To confirm that the late-flowering phenotype is due to the suppression of OsMDS50 gene expression, transgenic plants were generated by expressing RNAi constructs of the gene as shown in FIG. 3a. MAD S-box deleted OsMADS50 genes were cloned into pBluescript SK (−) vector (Stratagene) in opposite directions at both sides of the GUS gene, and then inserted in the pGA1611 vector (AY373338) at the position between the maize ubiquitin promoter (Pubi) and the nos terminator (Tnos). Among 82 T1 plants, 76 showed the delayed flowering phenotype delayed by at least one month (FIG. 3b). Six plants flowered 74 to 78 days after planting, similarly to the wild type control and transgenic controls; eight did not flower until 140 days after planting. These results show that the OsMADS50 gene is an important flowering activator.

[0101]In addition to the late-flowering phenotype, the transgenic plants carried more elongated internodes (FIG. 3c)....

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Abstract

The present invention relates to a flowering-time and / or stem elongation regulator isolated from rice, which is selected from OsMADS50, OsMADSS1, OsMADS56, OsMADS14, OsTRX1, OsVIN1, OsCOL4 and OsCOLS, a DNA construct containing the regulator, a transgenic plant, a part thereof, and plant cell transformed with the DNA construct, and method to control flowering-time and / or stem elongation using the regulator. In the present invention, the flowering-time and / or stem elongation can be controlled, and thereby, various agricultural benefits obtained.

Description

FIELD OF THE INVENTION[0001]The present invention relates to a regulator for flowering-time and / or internodes elongation, a transgenic plant transformed with the regulator, and a method to regulate flowering-time and / or stein elongation in plant.BACKGROUND OF THE INVENTION[0002]The growth phase of plants generally includes a vegetative growth phase and a reproductive growth phase. The transition from vegetative to reproductive growth is affected by various flowering signals. The flowering signals are affected by various factors, such as genetic factors such as genotype, and environmental factors such as photoperiod and light intensity, etc. (Dung et al., 1998; Yamamoto et al., 1998). The transition in the growth phase leads to various morphological changes of plant, which is interesting from a scientific viewpoint. Furthermore, due to the economic benefits gained by flowering regulation, many studies on flowering mechanisms have been carried out.[0003]In particular, molecular geneti...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C12N15/82C12N15/29A01H5/00A01H1/06C07K14/00
CPCC07K14/415C12N15/827C12N15/8261Y02A40/146C12N15/11C12N15/09C12N15/10
Inventor AN, GYNHEUNGLEE, SHINYOUNGJEONG, DONG-HOONYOO, JIHYERYU, CHOONG-HWANJEON, JONG-SEONGKIM, SUNG-RYULKIM, YOUNG-OCKKIM, JOONYULAN, SUYOUNGHAN, JONG-JINHAN, MIN-JUNG
Owner POHANG IRON & STEEL CO LTD
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