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Compositions containing platelet contents

a technology of platelet contents and compositions, which is applied in the field of compositions containing platelet contents, can solve the problems of not being able to generate primary tumor cultures with high efficiency, not being able to rapidly grow cultures for many applications, and not being able to meet the requirements of a large number of applications

Inactive Publication Date: 2011-07-14
MAYO FOUND FOR MEDICAL EDUCATION & RES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0008]The methods and materials provided herein can be used to grow adult stem cells rapidly, to differentiate stem cells (e.g., adult stem cells), to grow primary cell cultures (e.g., tumor cell lines), to grow tumor stem-like cells, and to identify effective growth factors. For example, the methods and materials provided herein can allow clinicians or medical personnel to develop patient-specific autologous cancer vaccines, while following FDA guidelines. Tumor cells such as malignant glioma cells grown using the methods and materials provided herein can maintain many aspects of neural tumor stem cell phenotypes and can be enriched in tumor-specific antigens desired for recognition of host immune responses. The growth kinetics of cells grown using the methods and materials provided herein can allow clinicians to manufacture sufficient cellular material for multiple vaccinations in a short time frame dictated by current standard therapeutic regimens. Thus, the methods and materials provided herein can provide additional options for the expansion and use of patient specific tumor material for cell therapy.

Problems solved by technology

Cells grown in this way can exhibit limited applicability as an effective antigen source because they often exhibit characteristics of differentiated glial cell subtypes with a reduced ability to recapitulate the original tumor in vivo.
These approaches do not appear to generate primary tumor cultures with high efficiencies and do not appear to allow for the growth of cultures fast enough for many applications.
While these methods can be used to generate cell cultures from malignant gliomas, these protocols typically include materials and methods not suitable for clinical use.

Method used

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  • Compositions containing platelet contents
  • Compositions containing platelet contents
  • Compositions containing platelet contents

Examples

Experimental program
Comparison scheme
Effect test

example 1

Source of Platelets

[0078]All donors donating apheresis platelets fulfilled eligibility criteria as defined by AABB Standards for Blood Banks and Transfusion Service and the Food and Drug Administration. Donors were screened using the Uniform Donor History Questionnaire (UDQ) and accompanying educational materials. This questionnaire is a screening document created by a coalition of regulatory, accrediting, and blood collecting institutions consisting of the Food and Drug Administration, Centers for Disease Control and Prevention, Armed Services Blood Program, National Heart Lung and Blood Institute, American Blood Resources Association, AABB, American Red Cross, and America's Blood Centers. Information concerning the UDQ can be found on the World Wide Web at “fda.gov / cber / dhq / dhq.htm.”

[0079]All apheresis platelet donations were tested with the following infectious disease tests: (1) Serologic test for syphilis; (2) HCV EIA-hepatitis C virus antibody test, (3) HCV NAT-hepatitis C vir...

example 2

Preparing Platelet Lysate from Apheresis Platelets

[0082]Apheresis platelets were obtained as described in Example 1. The apheresis platelets used were no more than four days past expiration. A single lot of platelet lysate consisted of ten individual apheresis platelet units, and one lot was used at a time to create a platelet lysate product. The processing for clinical grade reagents can be performed in a clean room suite. Ten individual apheresis platelet units were frozen at −70° C. or colder. After being frozen for at least 24 hours, the units were removed from the freezer and allowed to thaw. The units were thawed at room temperature or at refrigerated temperatures. After thawing was complete, each unit was mixed by massaging the bag. Each thawed platelet bag was placed flat (to minimize breakage of tubing) in a supercold freezer (−70° C. or colder) for a second freeze. After the apheresis platelet units were frozen for at least 24 hours for a second freeze, they were removed f...

example 3

Culturing Cells with Platelet Lysates

[0092]The phenotypic characteristics of GBM cells cultured with media containing platelet lysates were compared to those of GBM cells cultured using NSC media (Neurobasal media (Invitrogen, Grand Island N.Y.); recombinant EGF and FGF (R&D Systems, Minneapolis, Minn.), N2 and B27 supplements (Invitrogen, Grand Island N.Y.), glutamine and penicillin / streptomycin) or DMEM containing 10% FBS. The media containing platelet lysate (HCTL#3) consisted of Neurobasal media supplement with 5% platelet lysate, glutamine, and penicillin / streptomycin. Cells grown in NSC media formed classical neurospheres enriched in tumor stem cells (FIG. 1A). Cells grown in DMEM 10% FBS became adherent and were usually differentiated (FIG. 1A). Cells grown in HCTL#3 resulted in a mixed population of free-floating neurospheres and adherent neuropheres with superior growth kinetics (FIG. 1A and 1B). Cells cultured in HCTL#3 also exhibited many aspects of neural tumor stem cell...

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Abstract

This document provides methods and materials relating to platelet lysates. For example, methods and materials for using platelet lysate compositions to grow adult stem cells, to differentiate adult stem cells, to grow primary cell cultures, to grow tumor stem cells, and to identify effective growth factors are provided.

Description

BACKGROUND[0001]1. Technical Field[0002]This document relates to methods and materials involved in making and using growth factors, chemokines, and molecules responsible for growing, differentiating, or maintaining undifferentiated cells from normal human platelets (e.g., platelet lysates). For example, this document relates to methods and materials for manufacturing from platelets or platelet preparations (e.g., platelet apheresis preparations) those factors used to grow stem cells (e.g., adult stem cells) rapidly, to maintain them in an undifferentiated form, as an additive to media to differentiate stem cells (e.g., adult stem cells) in combination with other factors, to grow primary cell cultures (e.g., tumor cells and tumor cell lines), and to grow tumor cells with stem cell properties. This document also relates to methods and materials that can be used to identify, isolate, enrich, or optimize combinations of effective growth factors using platelets as a source material. In a...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C12N5/071C12N5/09C12N5/0775
CPCC12N5/0644A61K35/19C12N5/0037C12N5/0662C12N5/0693C12N2500/90
Inventor DIETZ, ALLAN B.GUSTAFSON, MICHAEL P.BUTLER, GREG W.
Owner MAYO FOUND FOR MEDICAL EDUCATION & RES
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