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Cancer tissue-derived cell mass and a process for preparing same

a cell mass and cancer tissue technology, applied in the field of cell mass, can solve the problems of difficult handling and difficult treatment of advanced cancers, and achieve the effect of simple and easy production of tumorigenic animals and excellent tumor settlemen

Inactive Publication Date: 2012-06-28
OSAKA PREFECTURAL HOSPITAL ORG +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

"The present invention provides a novel cell mass derived from a cancer tissue that can reconstruct in vitro the behavior of cancer cells in vivo and examine the effect of therapy correctly. This cell mass is useful as a sample for analysis and treatment of cancer, as it is available for easily producing a cancer animal model and has a sufficient settlement in a small amount when transplanted into different species of animals. The invention solves the problem of using heterogeneous cell lines as study materials for cancer research and provides a more reliable tool for cancer research and treatment."

Problems solved by technology

However, it is still difficult to treat advanced-stage cancers, and cancers have continued to occupy the first place of the Japanese cause of death.
However, as to the primary culture cell, its culture method has not been established yet, and its handling is difficult.
However, there is a negative report (Non-Patent Document 5) to the theory to explain the origin of cancer in terms of the stem cell in this way, and thus such a theory still remains a hypothesis.

Method used

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  • Cancer tissue-derived cell mass and a process for preparing same
  • Cancer tissue-derived cell mass and a process for preparing same
  • Cancer tissue-derived cell mass and a process for preparing same

Examples

Experimental program
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example 1

Preparation of Cell Mass Derived from Cancer Tissue of Colon Cancer Xenograft in Mice

[0112]The colon cancer xenografts in mice were prepared by a xenograft procedure as shown below.

[0113]At first a surgical resected specimen of a human tumor (colon cancer) is cut into small pieces (each about 2 mm cube) under aseptic conditions. Then, a small incision of about 5 mm was made at the back of mice (nude mice, preferably NOD / SCID mice) with a severe immunodeficiency, and a subcutaneous tissue is peeled from the animal. A tumor graft which has been prepared is subcutaneously inserted, and wound closure is performed with a skin suture clip. Some of the xenografts are observed as a subcutaneous tumor about 14 days later to three months later.

[0114]The produced mice bearing a colon cancer were bred under SPF (specific pathogen free) conditions, and when the tumor reached 1 cm in size, it is removed and collected into a 50 ml-centrifugal tube (IWAKI; 2345-050) containing 20 ml of DMEM (Gibco;...

example 2

Preparation of Cell Mass Derived from Cancer Tissue from Surgical Specimens of Human Colon Cancer

[0123]The cell mass derived from the cancer tissue was obtained in the same manner as in Example 1, except that surgical specimens of colon cancer were used. As a result, an almost sphere-shaped cell mass derived from the cancer tissue, similar to one as shown in FIG. 1, was obtained at least 12 hours later as shown in FIG. 7.

example 3

Preparation of Cell Mass Derived from Cancer Tissue from Surgical Specimens of Human Ovarian Cancer

[0124]The cell mass derived from the cancer tissue was obtained in the same manner as in Example 2, except that surgical specimens of ovarian cancer were used. As a result, an almost sphere-shaped cell mass derived from the cancer tissue, similar to one as shown in FIG. 1, was obtained at least 12 hours later as shown in FIG. 7.

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Abstract

Disclosed is a novel cell mass derived from a cancer tissue, which can reflect the in vivo behavior of a cancer cell correctly. Also disclosed is a process for preparing the cell mass. Specifically disclosed is a cell mass derived from a cancer tissue, which is an separated product that is isolated from a cancer tissue obtained from an individual as a mass containing at least three cancer cells or a cultured product of the separated product and which can retain a proliferation ability in vitro. The cell mass derived from a cancer tissue is produced by, for example, a preparation process comprising the steps of: treating a pulverized product of a cancer tissue removed from a living body with an enzyme; and selecting and collecting a mass containing at least three cancer cells among from an enzymatic treatment product.

Description

TECHNICAL FIELD[0001]The present invention relates to a cell mass derived from a cancer tissue (a cancer tissue-derived cell mass) and a process for preparing the same. More particularly, the present invention relates to a cancer tissue-derived cell mass , which is able to reconstruct a cancer in vitro, and retain a proliferation ability.BACKGROUND ART[0002]In recent years, therapeutic results of early-stage cancers have been drastically improved as a result of various studies that have been repeated to overcome cancers. However, it is still difficult to treat advanced-stage cancers, and cancers have continued to occupy the first place of the Japanese cause of death. According to vital statistics of 2007 by the Ministry of Health, Labour and Welfare, 340,000 people or more died of cancers a year.[0003]For cancer research so far, especially when examining its behavior in vitro, experiments using a cancer cell line that has been subcultured and established under optimized culture cond...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C12N5/02C12N5/00
CPCC12N2509/00C12N5/0693
Inventor INOUE, MASAHIROOHUE, MASAYUKI
Owner OSAKA PREFECTURAL HOSPITAL ORG