Kit for diagnosing prostate cancer and diagnosis method

a prostate cancer and kit technology, applied in the field of prostate cancer diagnostic kits and methods, can solve the problems of increasing the blood level of psa, requiring blood collection, and requiring diagnosis to be performed in hospitals, so as to facilitate collection and facilitate diagnosis of prostate cancer

Inactive Publication Date: 2012-10-04
CELL & BIO
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  • Abstract
  • Description
  • Claims
  • Application Information

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Benefits of technology

[0032]The kit and method for diagnosing prostate cancer according to the present invention use human urine as a sample, in which the human urine sample is easier to collect than blood which is used in conventional methods. In addition, if the amount of PSA in the urine of a subje

Problems solved by technology

However, when the prostate enlarges, a significant amount of PSA leaks out of the prostate.
In the case of prostate cancer, a large amount of PSA leaks out, thus in

Method used

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  • Kit for diagnosing prostate cancer and diagnosis method

Examples

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Effect test

example 1

Quantification of PSA in Human Urine Using Antibody to PSA

[0036]Urine was collected from each of 20 normal persons and 10 prostate cancer patients and centrifuged to remove the precipitate. PSA in the urine samples was quantified using ELISA (Abazyme cat no EL10005) in the following manner.

[0037]1) A plate in an ELISA kit was coated with an anti-human PSA monoyclonal antibody (cat no: MO-C40081C: C-PSA1, Abazyme, Needham, Mass., USA), and 50 μl of each of the samples was added to each well.

[0038]2) The sample in each well was agitated to spread, and then stored at 37° C. for 30 minutes.

[0039]3) Each well was washed 5 times with distilled water.

[0040]4) The sample in each well was treated with 100 μl of a horseradish peroxidase-conjugated secondary antibody (Abazyme, Needham, Mass., USA) and incubated at 37° C. for 30 minutes.

[0041]5) Each well was washed 5 times with distilled water.

[0042]6) The sample in each well was treated with 100 μl of a substrate solution, and then incubated ...

example 2

Quantification of PSA in Human Blood Using Antibody to PSA

[0046]3 ml of blood was collected from each of 20 normal persons and 10 prostate cancer patients and centrifuged at 10000 rpm for 5 minutes to precipitate the red blood cell clot, and the supernatant sera were separated. PSA in each serum was quantified using ELISA (Abazyme cat no EL10005) in the same manner as Example 1. The results of the measurement indicated that the average value of the amounts of PSA in the 20 normal persons was only 1.0615 pg / ml, whereas the amounts of PSA in the 10 prostate cancer patients varied in the range from 20 pg / ml to 800 pg / ml, and the average value thereof reached 332.5 pg / ml (FIG. 2).

[0047]From the results of Examples 1 and 2, it could be seen that, in the case of the prostate cancer patients, the amount of PSA in the blood significantly increased, whereas the secretion of PSA into the urine showed a tendency to decrease rather than increase. Thus, if the amount of PSA in the urine of a sub...

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Abstract

The present invention relates to a kit and method for diagnosing prostate cancer, which use an antibody to prostate-specific antigen (PSA) to detect PSA in human urine. More specifically, the invention relates to a kit for diagnosing prostate cancer, which comprises an antibody to PSA and uses human urine as a sample, and to a method for diagnosing prostate cancer, which comprises brining a human urine sample into contact with an antibody to PSA in order to detect PSA in the sample.

Description

TECHNICAL FIELD[0001]The present invention relates to a kit and method for diagnosing prostate cancer, which use an antibody to prostate-specific antigen.BACKGROUND ART[0002]Prostate cancer is the second leading cancer which causes the death of men in USA, and the number of prostate cancer patients in Korea is also increasing. Prostate cancer patients in Korea accounted for only 1.2% of male cancer patients in 1989, but account for 3% of male cancer patients at present. The mortality in prostate cancer patients in Korea in 1990 was only 0.2%, but was 1.6% in 2000. Unlike other cancers, prostate cancer grows very slowly. Accordingly, if prostate cancer can be diagnosed at an early stage, the possibility of treating it can be increased, suggesting that the early diagnosis of prostate cancer is important.[0003]Prostate specific antigen (PSA) is a serine protease that has a molecular weight of about 33 kDa and is expressed in the prostatic epithelium at a high level. It is secreted into...

Claims

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Application Information

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IPC IPC(8): G01N33/574C07K16/40
CPCG01N33/57434C07K16/18G01N33/5304
Inventor YOON, KANG JUNSON, YOUNG SOOKHONG, HYUN SOOKCHOI, HYEONGWON
Owner CELL & BIO
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