Crystal structures and methods using same

a crystal structure and crystal structure technology, applied in the field of molecular biology, can solve the problems of unclear whether, and achieve the effects of increasing fgfr3 expression and/or activity, promoting the formation of monomeric fgfr3, and reducing the amount of fgfr3 activity

Inactive Publication Date: 2012-12-20
GENENTECH INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

The anti-FGFR3 antibodies effectively inhibit FGFR3 activity, reducing tumor growth and proliferation in both multiple myeloma and bladder cancer models, offering a promising therapeutic approach by targeting the FGFR3 signaling pathway.

Problems solved by technology

However, it remains unclear whether FGFR3 signaling is indeed an oncogenic driver of in vivo growth of bladder tumors.

Method used

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  • Crystal structures and methods using same
  • Crystal structures and methods using same
  • Crystal structures and methods using same

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Materials and Methods

Cell Lines and Cell Culture

[0415]The cell line RT4 was obtained from American Type Cell Culture Collection. Cell lines RT112, OPM2 and Ba / F3 were purchased from German Collection of Microorganisms and Cell Cultures (DSMZ, (Germany)). Multiple myeloma cell line KMS11 was kindly provided by Dr. Takemi Otsuki at Kawasaki Medical School (Japan). Bladder cancer cell line TCC-97-7 was a generous gift from Dr. Margaret Knowles at St James's University Hospital (Leeds, UK). UMUC-14 cell line was obtained from Dr. H. B. Grossman (currently at University of Texas M.D. Anderson Cancer Center, TX). The cells were maintained with RPMI medium supplemented with 10% fetal bovine serum (FBS) (Sigma), 100 U / ml penicillin, 0.1 mg / ml streptomycin and L-glutamine under conditions of 5% CO2 at 37° C.

FGFR3S249C Dimerization Studies

[0416]UMUC-14 cells were grown in cysteine-free medium, treated with R3Mab or DTNB for 3 hr, and cell lysates were subject to immunoblot analysis under redu...

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Abstract

The present invention relates generally to the fields of molecular biology and growth factor regulation. More specifically, the invention concerns modulators of FGFR3 function, and the identification and uses of said modulators.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This application is a Continuation of U.S. application Ser. No. 12 / 661,852, filed Mar. 24, 2010 which claims priority to U.S. patent application No. 61 / 163,222, filed on Mar. 25, 2009, the contents of which are incorporated herein by reference.REFERENCE TO TABLE SUBMITTED[0002]This application is accompanied by a Table submitted in .txt format which contains the file titled “P4294R1-1C1 Table 6.txt”, the contents of which are incorporated in their entirety herein by reference. LENGTHY TABLESThe patent application contains a lengthy table section. A copy of the table is available in electronic form from the USPTO web site (). An electronic copy of the table will also be available from the USPTO upon request and payment of the fee set forth in 37 CFR 1.19(b)(3).FIELD OF THE INVENTION[0003]The present invention relates generally to the field of molecular biology. More specifically, the invention concerns anti-FGFR3 antibodies, and uses of sa...

Claims

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Application Information

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Patent Type & AuthorityApplications(United States)
IPC IPC(8): A61K38/54C12N9/12G06F19/16C12N9/96
CPCC07K16/2863C07K16/3038C07K2317/56C07K2317/565C07K2317/567C07K2317/73C07K2317/732C07K2317/77C07K2317/92C07K2319/30A61K39/3955C07K2317/34C07K2317/75A61K39/395A61K2039/505C07K2317/76C07K2317/24C07K2317/52A61K39/39558C07K16/3061C07K2317/55A61P19/00A61P35/00A61P37/00A61P43/00C07K16/28
InventorWIESMANN, CHRISTIAN
OwnerGENENTECH INC