Cumulative differential chemical assay identification
Inactive Publication Date: 2013-04-18
AZURE VAULT
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Benefits of technology
The present invention provides a method and apparatus for cumulative differential chemical assay identification, which involves measuring fluorescence values in a chemical reaction involving a test sample and a reference sample. Pairwise differences are calculated between the measured fluorescence values and the reference sample, and a cumulative index is calculated by adding together the pairwise differences based on their proximity to physical parameter values and difference size. The similarity between the test sample and the reference sample is determined based on the calculated cumulative index. The apparatus includes a value receiver and a difference calculator, while the method involves receiving fluorescence values, calculating pairwise differences, selecting a first difference, adding to it a plurality of others, and determining similarity. The technical effects of this invention include improved accuracy in identifying chemicals, more accurate matching of physical parameters, and faster and automated analysis of chemical reactions.
Problems solved by technology
Although asymmetric PCR generates brighter signals than symmetric PCR does, asymmetric PCR is seldom used because it is much less efficient than conventional PCR, as described in further detail hereinbelow.
While conventional symmetric PCR typically uses equimolar concentrations of two primers with similar melting points, conventional asymmetric PCR assays are inefficient and unpredictable, because they are designed using symmetric primers, without taking into account the effect of the actual primer concentrations on primer melting points.
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[0045]The present embodiments comprise methods and apparatuses for cumulative differential chemical assay identification.
[0046]Currently, comparisons made between fluorescence values measured during a chemical reaction (say LATE-PCR) which involves a test sample, and reference fluorescence values of a similar chemical reaction which involves a reference sample (typically of known content), often serve for identification of the test sample, as described in further detail hereinabove.
[0047]However, the currently serving comparisons are based on a pre-assumption that samples of similar content such as similar DNA sequences (say samples bearing a same mutation in a microorganism's DNA) yield similar fluorescence values for similar respective physical parameter values (say for a same temperature or pressure).
[0048]Consequently, the identification is typically based on calculation of differences between pairs of fluorescence values. Each pair pertains to a same value of the physical param...
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Abstract
An apparatus comprising: a value receiver, configured to receive fluorescence values measured during a chemical reaction involving a test sample, each value pertaining to a respective physical parameter value, a difference calculator, configured to calculate differences, each difference being between respective one of the measured fluorescence values and one of reference fluorescence values of a reference sample, each reference fluorescence value pertaining to a respective physical parameter value, a cumulative index calculator, configured to calculate a cumulative index, by selecting a first difference among the calculated differences, and selecting and adding to the first difference differences, each one of the added differences being selected according to a proximity standard applied on each two differences selected in a sequence, the proximity standard being based on proximity of physical parameter values and difference size, and a similarity determiner, configured to determine similarity between the samples, using the calculated cumulative index.
Description
FIELD AND BACKGROUND OF THE INVENTION[0001]The present invention relates to analyzing chemical assays and, more particularly, but not exclusively to cumulative differential photometric chemical assay identification.[0002]Some chemical assays can be identified by monitoring their varying photometric properties in an ongoing chemical reaction (say in an on going Quantitative Fluorescent Polymerase Chain Reaction (QF-PCR), on a DNA (Deoxyribonucleic acid) Melting Reaction, or in another chemical reaction), as known in the art.[0003]More specifically, crucial decisions in pre-implantation genetic diagnosis, infectious diseases, bioterrorism, forensics, and cancer research have increasingly depended on identification of specific DNA sequences, even down to alleles of single-copy genes in single cells.[0004]The identification typically involves introduction of fluorescently active agents that emit or quench fluorescent light when connected in a weak bond, say to a specific DNA sequence, w...
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Patent Timeline
Login to View More IPC IPC(8): G06F19/22G06F19/10G16B25/20
CPCG06F19/702G01N21/6428C12Q1/6816C12Q1/686G16B25/00G16C20/10G16B25/20C12Q2527/107C12Q2537/165C12Q2563/107C12Q1/6851G01N21/64
InventorRUSSAK, ZE'EV
OwnerAZURE VAULT