Recombinant phage and methods

a phage and phage technology, applied in the field of recombinant phage and methods, can solve the problems of limiting the natural host range of the phage engineered to date, affecting the engineering efficiency of the phage, and general lack of methods
US20130122549A1Active Publication Date: 2013-05-16INST FOR ENVIRONMENTAL HEALTH
2 Cites 38 Cited by

Patent Information

Authority / Receiving Office
US · United States
Patent Type
Applications(United States)
Current Assignee / Owner
INST FOR ENVIRONMENTAL HEALTH
Publication Date
2013-05-16

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

This disclosure provided methods of cloning a phage genome. Also provided are methods of making a recombinant phage genome. In some embodiments the phage genome is engineered to comprise a heterologous nucleic acid sequence, for example a sequence comprising an open reading frame. In some embodiments the phage genome is cloned in a yeast artificial chromosome. Recombinant phage genomes and recombinant phage are also provided. In some embodiments the methods are high throughput methods such as methods of making a plurality of recombinant phage genomes or recombinant phage. Collections of recombinant phage genomes and recombinant phage are also provided.
Need to check novelty before this filing date? Find Prior Art

Description

REFERENCE TO RELATED APPLICATIONS

[0001] This application claims priority to U.S. Provisional Patent Application No. 61 / 539,454, filed Sep. 26, 2011; U.S. Provisional Patent Application No. 61 / 549,743, filed Oct. 20, 2011; and U.S. Provisional Patent Application No. 61 / 642,691, filed May 4, 2012. The entire contents of each of those applications are hereby incorporated herein by reference.SEQUENCE LISTING

[0002] The instant application contains a Sequence Listing which has been submitted in ASCII format via EFS-Web and is hereby incorporated by reference in its entirety. Said ASCII copy, created on Nov. 14, 2012, is named 1211USNP.txt and is 12,065 bytes in size.INTRODUCTION

[0003] Model phage have been engineered using molecular biology techniques to deliver heterologous protein products to bacterial cells. For example, phage have been engineered to deliver enzymes to biofilms to digest the extracellular matrix and destroy the biofilm. (E.g., U.S. Patent Application Publication No. 2009 / ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More