Adeno-associated-virus rep sequences, vectors and viruses

a technology of vectors and viruses, applied in the field of adeno-associated virus (aav) replication (rep) sequences, can solve the problems of limited success in the prior art strategy of constructing hybrid viruses (e.g., ad/aav) by controlling rep expression, and achieve the effect of high level of rep protein expression
US20140155469A1Inactive Publication Date: 2014-06-05THE RES FOUND OF STATE UNIV OF NEW YORK
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Patent Information

Authority / Receiving Office
US · United States
Patent Type
Applications(United States)
Current Assignee / Owner
THE RES FOUND OF STATE UNIV OF NEW YORK
Publication Date
2014-06-05
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention provides adeno-associated virus (AAV) replication (Rep) sequences. In one embodiment, the invention provides nucleotide sequences encoding a chimeric protein, wherein the encoded chimeric protein contains a wild type AAV Rep inhibitory amino acid sequence, and wherein the nucleotide sequences contain a scrambled and / or deoptimized polynucleotide sequence encoding the wild type AAV Rep inhibitory amino acid sequence. The invention provides vectors, cells, and viruses containing the invention's sequences. Also provided are methods for detecting portions of the AAV Rep inhibitory amino acid sequence, which reduce replication and / or infection and / or productive infection by viruses. The invention's compositions and methods are useful for site-specific integration and / or expression of heterologous sequences by recombinant adeno-associated virus (rAAV) vectors and by rAAV virus particles, such as hybrid viruses (e.g., Ad-AAV) comprising such vectors. The invention's compositions and methods find application in, for example, gene therapy and / or vaccines.
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Description

[0001] This application claims priority under 35 U.S.C. §119(e) to co-pending U.S. Provisional Application Ser. No. 61 / 476,858, filed on Apr. 19, 2011, herein incorporated by reference in its entirety.

[0002] This invention was made with government support under grant AI41636, awarded by the National Institutes of Health (NIH). The government has certain rights in the invention.FIELD OF INVENTION

[0003] The invention provides adeno-associated virus (AAV) replication (Rep) sequences. In one embodiment, the invention provides nucleotide sequences encoding a chimeric protein, wherein the encoded chimeric protein contains a wild type AAV Rep inhibitory amino acid sequence, and wherein the nucleotide sequences contain a scrambled and / or deoptimized polynucleotide sequence encoding the wild type AAV Rep inhibitory amino acid sequence. The invention provides vectors, cells, and viruses containing the invention's sequences. Also provided are methods for detecting portions of the AAV Rep inhibito...

Claims

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