Method and compositions for consumables
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[0388]An exemplary muscle replica composition comprising one or more isolated, purified plant proteins is described herein.
[0389]Protein Purification for Components of the Replica
[0390]Moong bean seeds, Green Pea dry seed were purchased as milled flour and used for purification of respective seed storage proteins. Rubsico was purified from fresh alfalfa plant. Protein composition at individual fractionation steps was monitored by SDS-PAGE and protein concentrations were measured by standard UV-VIS and Pierce assay methods.
[0391]Moong bean 8S globulins: Moong bean flour was resuspended in in 50 mM potassium phosphate buffer pH 7 and 0.5M NaCl at 1:4 (wt / v) ratio, and mixture was incubated for 1 hr. Unsoluble material was separated by centrifugation and proteins in the supernatant were fractionated by addition of ammonium sulfate in 2 steps: 50% (wt / v) followed by 90% (wt / v). Protein precipitated in 90% fraction contained the moong bean 8S globulins and was stored at −20 C until furth...
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