Insecticidal compositions and methods of using the same
a technology of insecticides and compositions, applied in the field of insecticide compositions and methods useful for insect control, can solve the problems of many insecticide disadvantages, surface films may also impede adult mosquitoes from laying eggs, and many insecticides are difficult to develop resistan
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reference example 1
Materials and Methods
[0051]Reagents. Mineral oil was supplied by Brenntag Great Lakes, LLC (Wauwatosa, Wis.), Barton Solvents (Des Moines, Iowa), and Price Tech Group (Channahon, Ill.). Diluents were supplied by Stepan Company (Chicago, Ill.), Cognis Corporation (Ambler, Pa.), and Procter & Gamble Chemicals (Cincinnati, Ohio). Surface active agents were supplied by Akzo Nobel Surfactants (Chicago, Ill.), Stepan Company (Chicago, Ill.), Harcros Chemicals, Inc. (Joliet, Ill.), Huntsman Corporation (The Woodlands, Tex.), Croda Inc. (Edison, N.J.), and Dow Chemical (Midland, Mich.).
[0052]Laboratory Bioassay Method. All larvae were reared on a diet of ground TETRAMIN® fish food (Tetra, Blacksburg, Va.) in an insectary maintained at 30° C. and 45% relative humidity (RH). Mainly third instars of Aedes aegypti were used for the laboratory container experiments. Anopheles quadrimaculatus and Culex quinquefasciatus were also tested where indicated.
[0053]Laboratory bioassays were conducted in ...
example 2
Compositions Containing Six Different Lots of Mineral Oil
[0056]A 50.00 g sample of liquid larvicide was prepared by adding 49.37 g (98.75% wt) white mineral oil, 0.48 g (0.95% wt) of a 4-mole ethoxylated nonylphenol (WITCONOL™ NP 40, Akzo Nobel Surfactants, Chicago, Ill.), and 0.15 g (0.30% wt) silicone oil to a 6 oz glass jar. The sample was mixed with an overhead stirrer at medium speed for 15 min. All samples were prepared with different lots of mineral oil and the same lot of the nonylphenol and silicone oil. Samples were tested using the Laboratory Bioassay Method (Reference Example 1).
TABLE 1Comparative bioassay of six different lots of mineral oil on3rd instar Aedes aegypti at 21° C.% MORTALITYMINERAL OIL24 HAT48 HAT72 HATSample 1100100100Sample 2103347Sample 3318284Sample 463550Sample 5599396Sample 6378187* HAT = Hours After Treatment (hours after liquid larvicide (treatment) is applied to the surface of the water).
[0057]According to the results shown in Table 1, the composi...
example 3
Compositions Having Varying HLB Numbers
[0058]Surfactants with different HLB numbers were tested in an effort to reduce the variable efficacy of the compositions demonstrated in Example 2.
[0059]Consistent with the compositions tested in Example 2, a 50.00 g sample of liquid larvicide was prepared by adding 49.37 g (98.75%) of white mineral oil, 0.48 g (0.95%) of an ethoxylated nonylphenol (WITCONOL™ NP Series, Akzo Nobel Surfactants, Chicago, Ill.), and 0.15 g (0.30%) silicone oil to a 6 oz glass jar. The sample was mixed with an overhead stirrer at medium speed for 15 min. All samples were prepared with one lot of mineral oil, one lot of silicone oil, and an ethoxylated nonylphenol from the WITCONOL™ Series of surfactants with an increasing HLB number. In addition to the screening of each WITCONOL™ surfactant as illustrated in Table 2, a second series of blends of WITCONOL™ surfactants was also tested for biological activity, as shown in Table 3. Samples were tested using the Labora...
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