Use of ep4 receptor antagonists in the treatment of cartilage disease

a technology of ep4 receptor and cartilage disease, which is applied in the field of compounds, can solve the problems of no effect, no no anti-ep4 receptor, and all therapeutic methods are only nosotropi

Inactive Publication Date: 2016-11-03
ASKAT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patent describes the discovery of a compound that can inhibit the destruction of cartilage in several animal models. The results suggest that the compound may be effective in treating and preventing cartilage diseases in humans.

Problems solved by technology

And it undergoes, depending on the stage of the lesion, a hyperplasia of a synovial membrane, a destruction of a subcartilaginous bone, a hyperplasia or a neoplasia of a circumarticular cartilage, which are followed by a deformation of the cartilage, which may lead to dysfunction in a serious case.
However, all therapeutic methods are only nosotropic, and exhibit no sufficient effects.

Method used

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  • Use of ep4 receptor antagonists in the treatment of cartilage disease
  • Use of ep4 receptor antagonists in the treatment of cartilage disease
  • Use of ep4 receptor antagonists in the treatment of cartilage disease

Examples

Experimental program
Comparison scheme
Effect test

example 1

Ex Vivo Bovine Cartilage Explant Model

[0781]The method of this assay is descrived in the following literature (Rheumatol Int. 2013 February; 33(2):401-11).

[0782]Full-depth cartilage explants were harvested from the knee of either back legs of a cow less than 24 month old. All the FDC explants are divided into different treatment group according to the explants weight and metabolic activity (viability) measured by Alamar Blue. Compounds were freshly added to the explants at each media change. The explants were cultured in serum-free conditions. Supernatants retrieved from the culture were analyzed by biomarkers (P2NP, C2M, AGNx1, AGNx2) at day 7, 14 and 21.

[0783]These results are shown in FIG. 1 and FIG. 2.

[0784]From results of FIG. 1, Compound A and Compound B shows dose-dependent inhibition of cartilage destruction in ex vivo bovine cartilage explant model

[0785]The similar inhibition of cartilage destruction in ex vivo bovine cartilage explant modelis shown in Compound C[0786](4-{(...

example 2

Rat Mono-Iodoacetate and / or Meniscal Transection Model

[0791]The compounds described in the compounds list are conducted in the rat mono-iodoacetate and / or meniscal transection induced osteoarthritis model. These compounds exhibit potent inhibitory activities on cartilage destruction and serum bio-chemical markers associated with cartilage degradation and tissue inflammation in the rat mono-iodoacetate and / or meniscal transection model.

[0792]The study was performed in female Sprague-Dawley rats, age 6 months at MNX surgery.

[0793]7 days after MNX surgery monoiodoacetate 2 mg / 0.2 ml was injected intra-articularly in the right knee joint. The test compounds or vehicle is injected to the animals after the MNX. In the MNX or sham group, the test compounds or vehicle was administered until termination.

[0794]Weekly fasting blood sampling are taken for the analysis of biomarker (P2NP, C2M, AGNx1, AGNx2). Knee joints is isolated at termination. Cartilage damage is scored by experienced histo-...

example 3

Rat Meniscal Transection and / or Ovariectomised Model

[0796]The compounds describe in the compounds list are conducted in the rat meniscal (MNX) transection and / or ovariectomised (OVA) induced osteoarthritis model. These compounds exhibit excellent inhibitory activities on cartilage destruction and serum biochemical markers associated with cartilage degradation and tissue inflammation in the rat meniscal transection and / or ovariectomised model.

[0797]The study is performed in female Sprague-Dawley rats with MNX and OVX surgery.

[0798]The test compounds or vehicle is injected to the animals after the surgery. In the MNX / OVX or sham group, the test compounds or vehicle was administered until termination.

[0799]Weekly fasting blood sampling are taken for the analysis of biomarker (P2NP, C2M, AGNx1, AGNx2). Knee joints is isolated at termination. Cartilage damage is scored by experienced histo-pathologist according to Colombo score.

[0800]The compounds describe in the compounds list are simil...

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Abstract

This invention relates to a compound with EP4 antagonistic activity, or a pharmaceutically acceptable salt with EP4 receptor antagonistic activities, which is useful in the treatment of cartilage disease. This invention also relates to a compound of formula (I), (II), (III), (IV), (Va) or (Vb), or a pharmaceutically acceptable salt thereof with EP4 receptor antagonistic activities, which is useful in the treatment of cartilage disease. This invention also relates to a pharmaceutical composition for the treatment of cartilage disease which comprises a therapeutically effective amount of a compound of formula (I), (II), (III), (IV), (Va) or (Vb), or a pharmaceutically acceptable salt thereof. Further this invention relates to a method for the treatment of cartilage disease in an animal subject including a mammalian subject, which comprises administering to the animal subject including a mammalian subject a compound of the formula (I), (II), (IV), (Va) or (Vb), or a pharmaceutically acceptable salt thereof.

Description

TECHNICAL FIELD[0001]This invention relates to compounds for use in therapeutic treatment of the human body. In particular, it relates to compounds with selective EP4 receptor antagonism which are useful for treating cartilage disease, or preventing or delaying the onset or the progression of the said disease.[0002]This invention also relates to a pharmaceutical composition for the treatment of cartilage disease which comprises a therapeutically effective amount of a compound of formula (I), (II), (III), (IV), (Va) or (Vb), or a pharmaceutically acceptable salt thereof. This invention relates to a method for the treatment of cartilage disease in an animal subject including a mammalian subject, which comprises administering to the animal subject including a mammalian subject a compound of the formula (I), (II), (III), (IV), (Va) or (Vb), or a pharmaceutically acceptable salt thereof. Further this invention relates to a method for the treatment of cartilage disease in an animal subjec...

Claims

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Application Information

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IPC IPC(8): A61K31/4412A61K31/437A61K31/381A61K45/06
CPCA61K31/4412A61K31/381A61K31/437A61K45/06A61K31/44C07D333/38C07D471/04A61K31/443C07D213/82C07D235/18C07D405/12C07D409/10A61P19/02A61P19/08A61P29/00A61P43/00A61K31/341C07D213/89C07D307/64
Inventor OKUMURA, TAKAKO
Owner ASKAT
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