Delivery of payloads to stem cells

a stem cell and payload technology, applied in the direction of genital tract cells, skeletal/connective tissue cells, peptide/protein ingredients, etc., can solve the problems of difficult patient eating, severe side effects, and difficult for patients, especially children, to cope with

Pending Publication Date: 2020-12-31
GLADIATOR BIOSCI INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Benefits of technology

[0221]In one embodiment the chemotherapy combination comprises ganciclovir, which may assist in controlling immune responses and / or tumour vasculation.
[0232]In one embodiment, the formulation is for intravenous (i.v.) administration. This route is particularly effective because it allows rapid access to the majority of the organs and tissue and is particular useful for the treatment of metastases, for example established metastases especially those located in highly vascularised regions such as the liver and lungs.

Problems solved by technology

This “obliteration therapy” has many side effects, for example mouth and throat pain (which may make it difficult for the patient to eat), nausea and vomiting, susceptibility to infection, such as pneumonia and CMV infection, anemia, bleeding, infertility, cognitive dysfunction, etc.
These side effects are very severe, and are difficult for patients, especially children to cope with.
However, the treatment is reserved for only the severest of cases because the risk associated with the treatment are significant.

Method used

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  • Delivery of payloads to stem cells
  • Delivery of payloads to stem cells
  • Delivery of payloads to stem cells

Examples

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examples

[0245]FIG. 1A-D Shows various representations of GLA protein structures.

[0246]FIG. 1E Shows an embodiment of a GLA-component according to the present disclosure.

[0247]FIG. 2 Shows Protein S (PrS) and annexin staining of breast cancer cell lines treated with peroxide to induce apoptosis. A, human MDA-231 cells treated with peroxide and stained with FITC-PrS. B, untreated MDA-231 cells stained as in A. C, treated MDA-231 cells stained with annexin. D, human MCF-7 cells treated with peroxide and stained with PrS. E, murine MET-1 cells, as in D. F, murine 4T1 cells, as in D.

[0248]FIG. 3 Shows overlapping, yet distinct, cellular localization of PrS and annexin. A, murine 4T1 cells treated with peroxide and stained with Cy5 PrS (RED) and FITC annexin (GREEN). Light arrow, co-localized signals; red arrows, cells staining with PrS and not annexin; green arrow, cell staining relatively brighter with annexin but less bright with PrS, indicating distinct binding patterns (insets show PrS and a...

example 2

[0290]Stem cells are distinct in phenotype from differentiated cells and may express PS non-apoptotically to avoid the induction of immune responses. Stem cells were stained with a GLA domain molecule of the present disclosure comprising a payload of a fluorescent label, without the induction of apoptosis.

[0291]Trophoblast stem cells, (FIG. 14) which differentiate into several types of trophoblasts in the placenta, stained with Protein S, whereas differentiated trophoblasts derived from these cells in culture did not stain. The stain was able to distinguish between in vivo differentiated stems cells and cells differentiated in vitro.

[0292]This data the molecules of the present disclosure may be employed to target cells in vivo or in ex vivo samples.

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Abstract

The present disclosure relates to a method of targeting stems cells, in particular non-apoptotic stem cells, employing a GLA domain, capable of binding surface exposed phosphatidyl serine.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This application claims the benefit of U.S. Provisional Application No. 62 / 554,530 filed Sep. 5, 2017, U.S. Provisional Application No. 62 / 554,533 filed Sep. 5, 2017, U.S. Provisional Application No. 62 / 569,403 filed Oct. 6, 2017, U.S. Provisional Application No. 62 / 569,411 filed Oct. 6, 2017, U.S. Provisional Application No. 62 / 584,565 filed Nov. 10, 2017, and U.S. Provisional Application No. 62 / 593,014 filed Nov. 30, 2017, each of which applications is herein incorporated by reference in its entirety.INCORPORATION OF SEQUENCE LISTING[0002]This application contains a sequence listing submitted electronically via EFS-web, which serves as both the paper copy and the computer readable form (CRF) and consists of a file entitled “ST-CT1-PCT_sequence.txt”, which was created on Sep. 5, 2018, which is 9,831 bytes in size, and which is herein incorporated by reference in its entirety.[0003]The present disclosure relates to a method of targeting s...

Claims

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Application Information

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Patent Type & Authority Applications(United States)
IPC IPC(8): C07K14/745C12N5/0789C12N5/095
CPCC12N5/0647C12N5/0695C07K14/745A61K38/4833A61K38/4846A61K38/4866C12N5/0605C12N5/0631C12N5/0663C12N5/0682C12N5/0693C07K2319/035C12N2502/30Y02A50/30A61K45/06A61K38/1808C07K2319/60A61K49/0043A61K47/645A61K38/36
Inventor HERMISTON, TERRYBAUZON, MAXINECONTAG, CHRISTOPHER H.HARDY, JONATHANBLANKENBERG, FRANCIS GERARD
Owner GLADIATOR BIOSCI INC
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