Antiviral composition against flavivirus
a technology of antiviral composition and flavivirus, applied in the field of composition, can solve the problems of no approved antiviral therapy or treatment of most of the flavivirus infection, many deaths and afflicted millions of people, and threaten almost 2.5 billion people living, and achieve the effect of reducing the yield of the virus
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example a
[0063]Example A shows a composition of baicalein that exhibits prophylactic effects on dengue virus type-2 (DENV-2).
[0064]In order to determine the prophylactic anti-dengue activity of baicalein, different concentrations of baicalein (6.25 μg / ml, 12.50 μg / ml, 25.0 μg / ml, and 50.0 μg / ml) were added to the Vero cell monolayers five hours before adding virus. After five hours of pre-infection treatment, the cells were washed twice with phosphate-buffered saline (PBS). Then, 200 FFU (focus-forming units) of dengue virus type-2 (DENV-2) was inoculated to the cells to produce infected cells. The infected cells were incubated at 37° C. for one hour. Then the infected cells were washed two times with PBS to eliminate the unadsorped viruses. Growth medium (the growing medium is but not limited to Eagle's minimal essential medium; EMEM) was supplemented with 2% fetal bovine serum (FBS), 1 mM non-essential amino acid solution, 1 mM L-glutamine solution, and 1.5% carboxymethyl cellulose (CMC) s...
example b
[0066]Example B shows a composition of baicalein that shows inhibition of DENV-2 attachment to host cells.
[0067]Inhibition of DENV-2 attachment to host cells was evaluated by adding different concentrations (3.12 μg / ml, 6.25 μg / ml, 12.5 μg / ml, 25.0 μg / ml, and 50.0 μg / ml) of baicalein to Vero cells simultaneously with 200 FFU DENV-2. Then, the infected Vero cells were incubated at 37° C. for one hour in the presence of the respective concentration of baicalein. Following after, the infected Vero cells were washed two times using sterile PBS. Growth medium supplemented with 2% fetal bovine serum (FBS), 1 mM non-essential amino acid solution, 1 mM L-glutamine solution, and 1.5% carboxymethyl cellulose (CMC) solution) was added to the infected cells in the microplate. The microplate was incubated at 37° C. for four days. The number of viral foci formed and the viral RNA level were determined. The amount of viral RNA synthesis was determined using quantitative real time polymerase chain ...
example c
[0069]Example C shows a composition of baicalein that shows antiviral activity on dengue virus type-2 (DENV-2) replication when added post-infection.
[0070]In order to evaluate the antiviral activity of baicalein after virus attachment to cells, virus inoculum consisting of 200 FFU DENV-2 was added to the monolayer Vero cells and the virus was allowed to adsorp to the Vero cells for one hour at a temperature of 37° C. Unadsorped viruses were removed by rinsing the Vero cells with sterile PBS for two times. Different concentrations of baicalein (3.12 μg / ml, 6.25 μg / ml, 12.5 μg / ml, 25.0 μg / ml, and 50.0 μg / ml) were mixed with 1.5% CMC containing cell-growth medium supplemented with 2% FBS respectively before incubated at 37° C. for four days. Then, the number of viral foci and viral RNA level were measured.
[0071]FIGS. 3(a) and (b) showed that 50 μg / ml of baicalein reduced the number of dengue virus foci formed by 78.3% and decreased the level of DENV-2 RNA production by 84.9%±2.15 compa...
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