The invention provides a low-temperature storage system, which does not cause a temperature rise of a container in extraction action, prevents deterioration of a sample in the container and moisture condensation on the container, does not require a special machine, and can work at high speed, and costs required by manufacture and use are reduced and the system is easy to use. Specifically, a low-temperature storage system (100) has a container housing unit (110) which formed in a manner of being movable in a space at an upper side of a lower-temperature storage chamber (101) where a plurality of containers (C) housing samples are stored, and the container housing unit (110) has a low-temperature housing groove (120), an upper opening, and housing containers (C), and a coolant tube (121) which cools the inside of the low-temperature housing groove (120).