Acid soluble fish skin collagen and its preparing method
A fish skin collagen and collagen technology, applied in animal/human proteins, connective tissue peptides, specific peptides, etc., can solve the problem of acid-soluble collagen that has not been reported, and achieve white color, simple process, and collagen loss. small amount of effect
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Embodiment 1
[0031] Take 1Kg of frozen fish skin, thaw it, drain it, and cut it into smaller than 0.5cm 3 of pieces. Add 10L of 2% NaCl solution, soak at 4°C for 18h, then centrifuge at 3000rpm for 20min, and discard the turbid supernatant. Then put the fish skin into the mixture of 5 L of acetone and 5 L of ethanol that had been pre-cooled at -20°C. After 4 hours, the fish skin was taken out and dried in a fume hood. Then dissolve the fish skin in a mixture of 13.7L 0.1mol / L citric acid and 1.36L 0.5mol / L acetic acid (10:1 total 15L), soak at 4°C for 24h, centrifuge at 10000rpm for 40min at 4°C, and remove the precipitate Repeat this step to extract twice more, combine the supernatants, and store them at 4°C to obtain an acid-soluble collagen solution; directly add 1.75Kg of NaCl solid to the above solution to make the final concentration reach 1mol / L, carry out salting out for 4 hours, and then Centrifuge at 4°C, 6000rpm for 20min, redissolve the collagen precipitate in 0.5mol / L acetic...
Embodiment 2
[0033] Take 1Kg of frozen fish skin, thaw it, drain it, and cut it into smaller than 0.5cm 3of pieces. Add 10L of 4% NaCl solution, soak at 4°C for 20h, then centrifuge at 3000rpm for 20min, and discard the turbid supernatant. Then put the fish skin into a mixture of 7.5 L of acetone and 7.5 L of ethanol that had been pre-cooled at -20°C. After 8 hours of exposure, the fish skin was taken out and dried in a fume hood. Then dissolve the fish skin in a mixture of 17.5L 0.1mol / L citric acid and 2.5L 0.5mol / L acetic acid (7:1 total 20L), soak at 4°C for 22h, centrifuge at 12000rpm for 20min at 4°C, and remove the precipitate Repeat this step to extract once more, combine the supernatant, and store at 4°C to obtain an acid-soluble collagen solution; directly add 4.68Kg of NaCl solid to the above solution to make the final concentration reach 2mol / L, carry out salting out for 12h, and then Centrifuge at 4°C and 8000rpm for 15 minutes, redissolve the collagen precipitate in 0.5mol / ...
Embodiment 3
[0035] Take 1Kg of frozen fish skin, thaw it, drain it, and cut it into smaller than 0.5cm 3 of pieces. Add 20L of 5% NaCl solution, soak at 4°C for 12h, centrifuge at 4000rpm for 10min at 4°C, and discard the turbid supernatant. Then put the fish skin into the mixture of 12.5 L of acetone and 12.5 L of ethanol that had been pre-cooled at -20°C. After 12 hours of action, the fish skin was taken out and dried in a fume hood. Then dissolve the fish skin in a mixture of 5L 0.1mol / L citric acid and 5L 0.5mol / L acetic acid (1:1, 10L in total), soak at 4°C for 20h, centrifuge at 10,000rpm for 50min at 4°C, and obtain the supernatant , stored at 4°C to obtain an acid-soluble collagen solution; directly add 3.5Kg of NaCl solid to the above solution to make the final concentration reach 3mol / L, carry out salting out for 1h, and then freeze and centrifuge at 6000rpm at 4°C for 15min to reconstitute the collagen precipitate. Dissolve in 0.5mol / L acetic acid solution, then in 0.02mol / L ...
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