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Acid soluble fish skin collagen and its preparing method

A fish skin collagen and collagen technology, applied in animal/human proteins, connective tissue peptides, specific peptides, etc., can solve the problem of acid-soluble collagen that has not been reported, and achieve white color, simple process, and collagen loss. small amount of effect

Active Publication Date: 2007-10-31
DALIAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, an efficient process for extracting acid-soluble collagen from fish skin—especially sea fish—has not yet been reported.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Take 1Kg of frozen fish skin, thaw it, drain it, and cut it into smaller than 0.5cm 3 of pieces. Add 10L of 2% NaCl solution, soak at 4°C for 18h, then centrifuge at 3000rpm for 20min, and discard the turbid supernatant. Then put the fish skin into the mixture of 5 L of acetone and 5 L of ethanol that had been pre-cooled at -20°C. After 4 hours, the fish skin was taken out and dried in a fume hood. Then dissolve the fish skin in a mixture of 13.7L 0.1mol / L citric acid and 1.36L 0.5mol / L acetic acid (10:1 total 15L), soak at 4°C for 24h, centrifuge at 10000rpm for 40min at 4°C, and remove the precipitate Repeat this step to extract twice more, combine the supernatants, and store them at 4°C to obtain an acid-soluble collagen solution; directly add 1.75Kg of NaCl solid to the above solution to make the final concentration reach 1mol / L, carry out salting out for 4 hours, and then Centrifuge at 4°C, 6000rpm for 20min, redissolve the collagen precipitate in 0.5mol / L acetic...

Embodiment 2

[0033] Take 1Kg of frozen fish skin, thaw it, drain it, and cut it into smaller than 0.5cm 3of pieces. Add 10L of 4% NaCl solution, soak at 4°C for 20h, then centrifuge at 3000rpm for 20min, and discard the turbid supernatant. Then put the fish skin into a mixture of 7.5 L of acetone and 7.5 L of ethanol that had been pre-cooled at -20°C. After 8 hours of exposure, the fish skin was taken out and dried in a fume hood. Then dissolve the fish skin in a mixture of 17.5L 0.1mol / L citric acid and 2.5L 0.5mol / L acetic acid (7:1 total 20L), soak at 4°C for 22h, centrifuge at 12000rpm for 20min at 4°C, and remove the precipitate Repeat this step to extract once more, combine the supernatant, and store at 4°C to obtain an acid-soluble collagen solution; directly add 4.68Kg of NaCl solid to the above solution to make the final concentration reach 2mol / L, carry out salting out for 12h, and then Centrifuge at 4°C and 8000rpm for 15 minutes, redissolve the collagen precipitate in 0.5mol / ...

Embodiment 3

[0035] Take 1Kg of frozen fish skin, thaw it, drain it, and cut it into smaller than 0.5cm 3 of pieces. Add 20L of 5% NaCl solution, soak at 4°C for 12h, centrifuge at 4000rpm for 10min at 4°C, and discard the turbid supernatant. Then put the fish skin into the mixture of 12.5 L of acetone and 12.5 L of ethanol that had been pre-cooled at -20°C. After 12 hours of action, the fish skin was taken out and dried in a fume hood. Then dissolve the fish skin in a mixture of 5L 0.1mol / L citric acid and 5L 0.5mol / L acetic acid (1:1, 10L in total), soak at 4°C for 20h, centrifuge at 10,000rpm for 50min at 4°C, and obtain the supernatant , stored at 4°C to obtain an acid-soluble collagen solution; directly add 3.5Kg of NaCl solid to the above solution to make the final concentration reach 3mol / L, carry out salting out for 1h, and then freeze and centrifuge at 6000rpm at 4°C for 15min to reconstitute the collagen precipitate. Dissolve in 0.5mol / L acetic acid solution, then in 0.02mol / L ...

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Abstract

The acid soluble fish skin collagen is prepared with sea fish skin as material and through the steps of cutting, NaCl solution soaking to eliminate impurity, alcohol and acetone defatting, soaking in mixed citric acid-acetic acid solution to extract, centrifugally separating to obtain acid soluble fish skin collagen solution, solid NaCl salt separation to obtain acid soluble fish skin collagen precipitate, dissolving in acetic acid solution, dialysis in Na2HPO4 solution or ultrafiltering with hollow acetate fiber film of molecular weight greater than 60 KDa, and vacuum freeze drying to obtain the product. The product is white, without fishy smell and spongy, and has purity over 97 %. It contains rich glycin, proline and hydroxyproline, as well as physiologically active peptide in its hydrolysate, and the collagen has molecular weight greater than 60 KDa.

Description

technical field [0001] The invention relates to the extraction of acid-soluble collagen and a preparation method thereof, which belongs to the field of biological products, by utilizing fish skin, which is a waste processed by seawater fish. Background technique [0002] Collagen is a structural protein that constitutes animal support tissues. Its products have been widely used in many fields such as medicine, health care, food processing, and cosmetics. So far, its products are mainly extracted from the skin of some terrestrial mammals such as cattle and pigs. In recent years, due to the impact of mad cow disease and foot-and-mouth disease, people began to seek safe, hygienic and harmless collagen extraction from aquatic animals such as fish, which has become a hot topic in research in various countries in recent years. Therefore, the extraction and utilization of collagen from fish processing waste can not only promote the comprehensive utilization of processing waste, tur...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/78
Inventor 朱蓓薇董秀萍刘兆芳闫雪徐美玲孙玉梅
Owner DALIAN POLYTECHNIC UNIVERSITY
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