Submerged solid fermentation method of fungus fermentation lacquer producing enzyme and its device

A solid fermentation device and solid fermentation technology, applied in the direction of fungi, oxidoreductase, etc., can solve the problems of unfavorable growth of microorganisms, reduce the use volume of fermenters, etc., and achieve the effects of improving gas exchange efficiency, saving equipment costs, and facilitating operation

Inactive Publication Date: 2008-02-27
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of this solid fermentation equipment are: (1) mechanical agitation is unfavorable to microbial growth, (2) easy to produce bacterial contamination, (3) reduces the actual use volume of the fermenter

Method used

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  • Submerged solid fermentation method of fungus fermentation lacquer producing enzyme and its device

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] As shown in Figure 1, the submerged solid fermentation device for producing laccase by fungus fermentation includes a fermenter 1, a vacuum pump 3, an oxygen cylinder 5, a steam generator 17, and a control system. The fermenter 1 is a constant temperature tank with a volume of 0.020 to 1.0 m 3 , its upper part is connected with the vacuum pump 3, its lower part is connected with the oxygen cylinder 5 and the steam generator 17 respectively, and the control system is connected with the fermenter 1, the vacuum pump 3 and the oxygen cylinder 5 respectively.

[0028] The vacuum pump 3 is connected to the upper part of the fermentation tank 1 through the relief solenoid valve 16 and the exhaust solenoid valve 14, and the oxygen cylinder 5 is connected to the bottom of the fermentation tank 1 through the pressure reducing valve 13, the inflation solenoid valve 15, and the gas filter 8 in turn, and the relief solenoid valve 16 , suction solenoid valve 14, and inflation solenoi...

Embodiment 2

[0035] The submerged solid fermentation method of producing laccase by fungus fermentation, the steps are:

[0036] (1) Pick 5 slices of mycelium pieces with a diameter of 1cm from the plate culture fungal strains (Gerium in white rot fungus), and insert them into 100 ml of synthetic liquid medium (add inorganic salts to 1 liter of potato juice). salt, 5 grams of potassium dihydrogen phosphate, 0.5 grams of magnesium sulfate, and 1 gram of calcium chloride), after 5 days of shaking culture at 28 ° C, transfer to a synthetic liquid medium filled with 1000 ml, and shake it for 3 days to obtain bacterial species liquid, i.e. the mycelia liquid used for expanding the inoculation;

[0037] (2) Put the wheat straw into the fermentation tank, generate steam through the steam generator and pass it into the fermentation tank, the steam pressure is 1.0kg / cm 2 , the time is 40min to sterilize the inside of the fermenter and the wheat straw;

[0038] (3) Take out the sterilized wheat st...

Embodiment 3

[0042] The submerged solid fermentation method of producing laccase by fungus fermentation, the steps are:

[0043] (1) Pick 5 slices of mycelium blocks with a diameter of 1em from the plate culture fungal strains (Germia in white rot fungi), and insert 100 ml of synthetic liquid medium (in 1 liter of potato juice, add inorganic salt, 5 grams of potassium dihydrogen phosphate, 0.5 grams of magnesium sulfate, and 1 gram of calcium chloride), and after 5 days of shaking culture at 28 ° C, transfer to a synthetic liquid medium containing 1000 ml, and then shake it for 3 days to obtain strains liquid, i.e. the mycelia liquid used for expanding the inoculation;

[0044] (2) Put the straw into the fermentation tank, generate steam through the steam generator and pass it into the fermentation tank, the steam pressure is 1.0kg / cm 2 , the time is 40min to sterilize the inside of the fermenter and the straw;

[0045] (3) Take out the sterilized straw from the fermenter, and mix the ba...

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Abstract

The invention offers a deep layer solid fermentation method of fungi fermenting producing laccase. The making steps are as follows: pre-culturing fungi in synthetic fluid culture medium to ferment to gain fungus stud-fluid; mixing with sterilized grass biomass material; putting in fermenter to ferment; doing intermittent exhausting-airing oxygen cycle ventilating operation; after fermenting, taking out material; extracting enzyme solution. The device includes fermenter, vacuum pump, oxygen bottle, steam generator, and control system. The fermenter is constant temperature jar; its upper is connected to the vacuum pump; its down part is respectively connected to the oxygen bottle, steam generator. The control system is respectively connected to the fermenter, the vacuum pump, and the oxygen bottle. The invention improves gas exchange efficiency and microorganism producing enzyme; it solves the problem of fungi pollution. Its device is simple, and can be operated automatically. It improves fermenting material burden, and realizes high yield and high efficiency of the fungi fermenting producing laccase.

Description

technical field [0001] The invention relates to the technical field of fungal fermentation, in particular to a submerged solid fermentation method for producing laccase by fungal fermentation and a device thereof. Background technique [0002] Many biological enzymes are produced by microorganisms through solid fermentation. Fungi and all aerobic microorganisms need oxygen in the process of growth and metabolic enzyme production. Microorganisms will produce carbon dioxide during the metabolic process. Since carbon dioxide is more specific than air and oxygen, the concentration of carbon dioxide produced around the fermentation raw materials is relatively high. Although the introduction of oxygen or increasing the oxygen pressure can increase the contact between oxygen and microorganisms, it still Can not fully penetrate the solid raw materials used in fermentation, especially for large solid fermentation equipment. In the solid fermentation device currently used, a mechanic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/02C12N1/14
Inventor 付时雨莫佳琳詹怀宇
Owner SOUTH CHINA UNIV OF TECH
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