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Medicinal composition, and its preparing method and use

A composition and drug technology, which can be applied in the directions of drug combinations, pharmaceutical formulations, active ingredients of heterocyclic compounds, etc., can solve the problems of low ganglioside content and the like

Active Publication Date: 2008-07-09
吉林天成制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0018] The contents of gangliosides in the currently disclosed brain extracts are all low, and although there are also public reports of gangliosides as components of the composition, and pharmaceutical compositions that work synergistically with other components, there is still no It involves the combination of venaolutong and animal brain extract (containing active polypeptides, various amino acids) and gangliosides to prepare new drugs

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0084] Take 10kg of fresh healthy bovine brain, remove impurities such as fascia, wash with water for injection, add 10L water for injection, homogenate, heat at 80°C, keep for 15 minutes, cool to 42°C, adjust pH value to 7.5, add 45g trypsin (USA DIFCO company, specification: 500g) was enzymatically hydrolyzed at 42°C for 3 hours, the pH value was controlled at 8.5 during the enzymolysis process, taken out, heated to 80°C, and kept for 20 minutes to obtain an enzymatic hydrolyzate;

[0085] After 24 hours at 4°C, centrifuge at 4,000 rpm for 15 minutes, filter the pulp, adjust the pH of the supernatant to 3.5, heat at 95°C, keep for 20 minutes, and centrifuge at 4°C for 12 hours. The condition is 4000 rpm, the centrifugation time is 20 minutes, the pulp is filtered, the filtrate is adjusted to pH 6.5, heated to 90°C, kept for 15 minutes, and then centrifuged at 8°C for 8 hours, the centrifugation condition is 4000 rpm, and the centrifugation time is In 20 minutes, the filtrate...

Embodiment 2

[0092] Take 10kg of fresh healthy pig brain, remove impurities such as fascia, wash with water for injection, add 10L water for injection, homogenate, adjust the pH value to 8.2, heat at 80°C, keep for 15 minutes, cool to 42°C, add 80g trypsin (U.S. DIFCO company, specification: 500g) was enzymatically hydrolyzed at 42°C for 10 hours, the pH value was controlled to 8.2 during the enzymolysis process, taken out, heated to 80°C, and kept for 20 minutes to obtain an enzymatic hydrolyzate;

[0093] Lower the temperature of the primary enzymatic hydrolysis solution to 44°C, adjust the pH value to 2.5, add 50 g of pepsin (Xiamen Xinglongda Chemical Reagent Co., Ltd; 1:10000; specification: 100 g), and enzymatic hydrolysis for 10 hours, the process pH value is controlled at 2.5 , and then heated to 90°C for 20 minutes to obtain a secondary enzymolysis solution;

[0094] Centrifuge at a speed of 4000 rpm for 30 minutes, filter the supernatant from a clarification plate, adjust the pH ...

Embodiment 3

[0101] Take 10kg of fresh healthy bovine brain, remove fascia and other impurities, wash with water for injection, add 25L water for injection, homogenate, adjust the pH value to 9.0, heat at 95°C, keep for 30 minutes, cool to 38°C, add 150g of trypsin, keep warm 4 hours, control the pH value to 8.0 during the enzymolysis process, then heat to 85°C and keep for 30 minutes to obtain an enzymolysis solution;

[0102] Place at 8°C for 8 hours, centrifuge at 4000 rpm, centrifuge for 20 minutes, filter the supernatant pulp, adjust the pH of the filtrate to 4.0, heat at 80°C, keep for 30 minutes, place at 5°C for 12 hours, centrifuge, The centrifugation condition is 4000 rpm, the centrifugation time is 30 minutes, the supernatant is filtered, the filtrate is adjusted to pH 7.0, heated to 95°C, kept for 30 minutes, and centrifuged at 7°C for 18 hours, the centrifugation condition is 4000 rpm, The centrifugation time is 30 minutes, and the supernatant is filtered to obtain a filtrate;...

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Abstract

The present invention discloses a medicine composition. Said medicine composition contains troxerutin obtained by making rutin undergo the process of hydroxyethylation, brain extract extracted from brain tissue of mammal except for human being and ganglioside extracted from brain tissue of mammal except for human being. Said invention also provides the concrete mixing ratio of the above-mentioned three components, and provides its preparation process and application.

Description

technical field [0001] The present invention relates to a pharmaceutical composition, its preparation method and its use, in particular, to a brain extract extracted from the brain tissue of mammals other than humans, venaorutong and containing A pharmaceutical composition composed of gangliosides extracted from mammalian brain tissue, its preparation method and its use in the preparation and treatment of acute and chronic cerebrovascular diseases, senile dementia, craniocerebral trauma, cerebrovascular accident trauma and post-trauma The use of sequelae drugs such as neurological sequelae and brain dysfunction caused by cerebrovascular diseases. Background technique [0002] Vinaolutong (also known as troxerutin) is a water-soluble flavonoid compound obtained by semi-synthesis of rutin through hydroxyethylation. D)-based mixture of hydroxyethyl rutin, generally used for medicine, calculated as anhydrous substance, containing troxerutin (C 33 h 42 o 19 ) shall not be les...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K35/30A61P25/28A61P25/00A61P9/10A61K31/37A61K31/7032
Inventor 彭瑞平
Owner 吉林天成制药有限公司
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