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Plasmin producing strain and method for producing plasmin

A technology of fibrinolysis and strains, applied in the direction of microorganism-based methods, biochemical equipment and methods, enzymes, etc., can solve the problems of high price, shortage of sources, and difficulty in exerting drug effects, and achieve low cost and strong feasibility Effect

Inactive Publication Date: 2008-08-13
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The research and development of thrombolytics is quite active, but the clinically used thrombolytics have certain defects in varying degrees: either strong toxicity and large side effects; or short half-life in the body, difficult to exert drug effects; shortage, expensive

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Bacillus sp.nov. SK006 was fermented under the following conditions,

[0021] Seed culture: seed culture medium (by mass volume percentage): peptone 1%, glucose 1%, yeast extract 0.5%, Na 2 HPO 4 12H 2 O 1.2%, MgSO 4 ·7H 2 O 0.03%, adjusted to pH 7.2, sterilized at 121°C for 20min, 180rpm, cultured at 37°C for 16-18h.

[0022] Liquid fermentation culture: liquid fermentation medium (by mass volume percentage): peptone 1.5%, glucose 1.5%, Na 2 HPO 4 12H 2 O 1.5%, NaH 2 PO 4 2H 2 O 0.13%, MgSO 4 ·7H 2 0.1%, antifoaming agent polyoxyethylene oxypropylene triglyceride 0.03%, 121 ℃ of sterilization 20min, adjust pH 7.2; Seed culture medium is inserted 25 liters of fermentation tanks by volume percentage 5% (filling capacity 15 liters ) liquid fermentation medium, the culture conditions are: stirring speed 180rpm, control ventilation: 0-12h is 9L / min, 12-24h is 10.5L / min, 24-36h is 12L / min; culture temperature is 37°C; After 36 hours, the enzyme activity of the f...

Embodiment 2

[0024] Bacillus sp.nov. SK006 was fermented under the following conditions,

[0025] Seed culture: seed culture medium (by mass volume percentage): peptone 1%, glucose 1%, yeast extract 0.5%, Na 2 HPO 4 12H 2 O 1.2%, MgSO 4 ·7H 2 O 0.03%, adjusted to pH 7.2, sterilized at 121°C for 20min, 180rpm, cultured at 37°C for 16-18h.

[0026]Liquid fermentation culture: liquid fermentation medium (by mass volume percentage): peptone 2.5%, glucose 2.5%, Na 2 HPO 4 12H 2 O 2.5%, NaH 2 PO 4 2H 2 O 0.13%, MgSO 4 ·7H 2 0.1%, antifoaming agent polyoxyethylene oxypropylene triglyceride 0.03%, 121 ℃ of sterilization 20min, adjust pH 7.2, seed culture medium is inserted 25 liters of fermentation tanks by volume percentage 5% (filling capacity 15 liters ) liquid fermentation medium, the culture conditions are: stirring speed 180rpm, control ventilation: 9L / min for 0~12h, 10.5L / min for 12~24h, 12L / min for 24~40h; culture temperature 40℃; After 40 hours, the enzyme activity of the fer...

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Abstract

The present invention relates to plasmin producing strain and method of producing plasmin, and belongs to the field of food biotechnology. The present invention screens out one plasmin strain with thrombolysis capacity from fermented food shrimp paste, and the new strain is identified as one new variety of bacillus, and named as Bacillus sp.nov.SK006 and has the preservation number of CCTCC No. M205071. The present invention also provides method of producing plasmin with the said strain, and the method has less breeding work, low cost, high safety and reliability and may be used in large scale liquid fermentation production. The fermented product is non-toxic, and may be used in preparing capsule, granule, oral liquid or oral lozenge or as additive for health food or common food.

Description

technical field [0001] A strain for producing fibrinolytic enzymes and a method for producing fibrinolytic enzymes. The present invention relates to a strain for producing fibrinolytic enzymes——Bacillus sp.nov. SK006, whose preservation number is CCTCC NO: M 205071, It belongs to the field of food biotechnology. Background technique [0002] Cardiovascular and cerebrovascular diseases have always been known as the number one killer threatening human health. According to relevant survey reports, one Chinese dies of cardiovascular and cerebrovascular diseases every 24 seconds, and one Chinese becomes disabled every 13 seconds. There are more than 3 million people who die from cardiovascular and cerebrovascular diseases in my country every year, accounting for 50% of the total number of deaths in my country every year. Among the people who survived the disease, 75% lost their ability to work to varying degrees, and 4% were seriously disabled. Thromboembolic disease is the numb...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N9/68C12R1/07
Inventor 江波峰芳德华颖王璋
Owner JIANGNAN UNIV
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