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Method for preparing tikitiki hemicellulose B

A technology for rice bran hemicellulose and defatted rice bran, which is applied in the field of preparing rice bran hemicellulose B, can solve the problems of browning of hemicellulose, cannot be used, browning of RBHB, etc., and achieves the effect of extensive physiological effects

Inactive Publication Date: 2008-11-19
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Researchers in Japan and other countries generally use the method of direct alkaline extraction and then use trichloroacetic acid to remove protein. After we practiced this method, we found that due to the large amount of rice bran protein during alkaline extraction, it will cause serious browning of hemicellulose, while If most of the proteins were removed by mild enzymatic method with alkali in advance, the proteins or amino acids involved in non-enzymatic browning were reduced a lot. The results confirmed that the RBHB obtained by the method they used had severe browning, and even could not Applications in Food and Beverage

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Dissolve 100 grams of defatted rice bran in 2-5L 0.2M acetic acid-sodium acetate buffer solution (PH4.8) containing 0.6% (V / V) high temperature resistant glucoamylase, keep it at 60°C for 24hr, centrifuge and place the residue in In 2-5L of 0.2M acetic acid-sodium acetate buffer solution (PH6) containing 1% (W / V) papain, keep 3hr at 60°C, repeat this operation after filtering and washing, then add 2-5L mass concentration of 4% NaOH solution in N 2 Alkaline extraction at room temperature under flow for 18 hours, after centrifugation, neutralize the extract with acetic acid to make the pH of the solution 5.0, remove the residue by centrifugation, dialyze the separated liquid under running water for 3 days, and then add 95% ethanol precipitation with 4 times the volume of the remaining dialysate , and finally the precipitate was dried at 60° C. for 16 hr, and finely ground to obtain 6.5 g of RBHB preparation.

Embodiment 2

[0022] Dissolve 1000 grams of defatted rice bran in 10 L of 0.2 M acetic acid-sodium acetate buffer solution (PH4.8) containing 0.6% (V / V) high-temperature resistant glucoamylase, keep it at 60°C for 24 hours, and after centrifugation, place the residue in a place containing 1 In the 0.2M acetic acid-sodium acetate buffer (PH6) of 10L of % (W / V) papain, keep 3hr at 60 ℃, repeat this operation after filtering and washing, then add 10L mass concentration and be 4% NaOH solution, in N 2 Alkaline extraction at room temperature under flow for 18 hours, after centrifugation, neutralize the extract with acetic acid to make the pH of the solution 5.0, remove the residue by centrifugation, dialyze the separated liquid under running water for 3 days, and then add 95% ethanol precipitation with 4 times the volume of the remaining dialysate , and finally the precipitate was dried at 60° C. for 16 hr, and finely ground to obtain 70 g of RBHB preparation.

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PUM

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Abstract

The invention relates to the manufacture method for rice bran hemicellulose B complex polysaccharide. It uses enzyme method and chemical method to make degrease rice bran hemicellulose B. Removing the starch, albumen, cellulose and semicellulose A, the rice bran hemicellulose B complex polysaccharide would be gained. The product has low viscosity, good swelling power and retention ability, and diet fiber content is over 93.57%.

Description

technical field [0001] The invention relates to a method for preparing hemicellulose, in particular to a method for preparing rice bran hemicellulose B. Background technique [0002] In the 1960s, Hipsley, Trowell, Cleave, and Burkitt successively reported the correlation between dietary fiber content in food and the incidence of Western "civilized diseases", and all pointed out that dietary fiber has important physiological functions on the human body, causing human Re-understanding of the role of dietary fiber (originally called "crude fiber", Crude fiber, now renamed Dietary fiber). In the past ten years, dietary fiber has become a hot research topic in professional fields such as food, nutrition, biochemistry and clinical medicine. In the food market of these countries, the sales of food and medicine related to dietary fiber have increased greatly. After more than 20 years of research and development, dietary fiber has become an important ingredient of health food widel...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/14
Inventor 胡国华盛春
Owner SHANGHAI NORMAL UNIVERSITY