An electrochemical current measurement device applicable to capillary electrophoresis
A current measuring device, a technology of capillary electrophoresis, which is used in measuring devices, scientific instruments, material analysis by electromagnetic means, etc., can solve problems such as sensitivity reduction, and achieve the effect of improving sensitivity and resolution
Inactive Publication Date: 2008-11-26
ZHEJIANG UNIV
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Problems solved by technology
However, when the sample to be tested enters the solution pool from the end of the capillary, the sample to be tested has somewhat diffused, so compared with the measurement inside the capillary, the sensitivity of the measurement will naturally decrease.
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[0023] As shown in Fig. 2, a kind of electrochemical current measuring device applicable to capillary electrophoresis comprises capillary 12 and solution pools 11 and 15 at both ends thereof, an electrophoretic voltage is added between the solution pools 11 and 15 at both ends, and the inner end of capillary 12 Place two distances near the solution pool 15 and be the working electrode 13 and the potential measurement electrode 14 of 500 nanometers-100 microns, the working electrode 13 and the potential measurement electrode 14 are connected with a potentiostat circuit 10; A voltage follower 17, an inverter 19, an adder 22, a potentiostat 16, and a programmable amplifier 18 connected together are composed of several resistors R1, R2, R3, R4, R5, and R6. The inverter 19 and the adder 22 There is a voltage input branch with an input terminal 21 between them, and the programmable amplifier 18 has an output terminal 20; the potential measuring electrode 14 is connected to the positi...
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Abstract
The invention discloses an electrochemical current measuring device for capillary electrophoresis, which comprises a capillary and solution pools of two ends, wherein the electrophoresis electric voltage is added between the solution pools of the two ends; two compact near working electrodes and a potential measuring electrode is positioned near the solution pool and on the end of the capillary; the working electrode and the potential measuring electrode are connected with a perpetuity electric potential apparatus circuit is formed by the in turn series connected voltage follower, changer, accumulator, perpetuity electric potential apparatus and the programmable amplifier matching a plurality of resistances; the voltage input sub circuit with input end is positioned between the changer and the accumulator; the programmable amplifier has an output end; the electric potential measuring electrode is connected with the positive of the follower; the working electrode is connected with the inverted output end of the perpetuity electric potential apparatus.
Description
technical field [0001] The invention relates to an electrochemical current measuring device, in particular to an electrochemical current measuring device applied to capillary electrophoresis. Background technique [0002] Capillary electrophoresis is an effective analytical chemical detection method. In the process of capillary electrophoresis, a high-voltage electric field is applied to the solution pool at both ends of the capillary, and various charged particles in the solution move under the action of this electric field. Various ions have different volume / charge ratios, and their movement speeds in the electric field are also different, so as to achieve the purpose of separation. After the various components in the sample to be tested are separated, appropriate detection means are required to detect them. At present, the most commonly used detection method in capillary electrophoresis is optical method, which includes laser-induced fluorescence detection method, ultra...
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IPC IPC(8): G01N27/447
Inventor 吴坚于吉人叶璟
Owner ZHEJIANG UNIV