A plant anti-reverse related protein, coding genes and an application thereof
A plant stress resistance and related protein technology, which is applied to the plant stress resistance related protein and its encoding gene and application field, can solve the problems of crop yield reduction, affecting plant growth and development, etc.
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[0033] Example 1. Acquisition and functional verification of rice stress-resistance-related proteins and their coding genes
[0034] 1. Acquisition of stress-related proteins and their coding genes in rice
[0035] Through yeast two-hybrid technology, rice stress-related protein TCTP was screened. Yeast two-hybrid system (CLONGTECH company) is a molecular biology method for studying protein-protein interactions. It is based on the fact that transcription factors can be divided into independent regions, namely DNA-binding domains and transcription activation domains, to achieve artificial simulation of transcription natural process. The bait protein used is DREB transcription factor, namely drought response element binding protein, which can specifically bind to the DRE / CRT cis-element in the promoter, activate the expression of many stress-induced genes, and enhance the tolerance of plants to adversity. Screening of proteins interacting with DREB transcription factors by yea...
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