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High frequency plant stem regeneration method by cell-culture of Xiangcao grass

A cell culture and plant technology, applied in plant regeneration, botanical equipment and methods, plant cells, etc., can solve the problem of low differentiation rate of green seedlings, inappropriate use of large-scale test-tube seedling propagation and molecular biology breeding, and loss of plant differentiation ability. And other issues

Inactive Publication Date: 2009-04-01
JIANGSU ACAD OF AGRI SCI +1
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  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Technical Problem The purpose of the present invention is to establish the technology of obtaining granular embryogenic callus and high-frequency plant regeneration by culturing young ears of Elephant Grass, so as to overcome the induction of white, translucent, sticky callus in tissue culture of Sorghum crops, The differentiation rate of green seedlings is low, and the callus loses the ability of plant differentiation after subculture, so it is not suitable for large-scale test-tube seedling propagation and molecular biology breeding.

Method used

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  • High frequency plant stem regeneration method by cell-culture of Xiangcao grass
  • High frequency plant stem regeneration method by cell-culture of Xiangcao grass
  • High frequency plant stem regeneration method by cell-culture of Xiangcao grass

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Embodiment Construction

[0024] 1 Materials and methods

[0025] 1.1 Materials

[0026] Elephant grass variety N51, (publicly known and public, introduced from the United States in 1985) is preserved by vegetative propagation of the seed stem, and expanded in the field. Cover with a black plastic film for 15 hours of dark treatment (9 hours of light during the day), and after 3 weeks of dark treatment, take the young ears of Elephant grass for in vitro culture.

[0027] 1.2 Medium

[0028] Minimal medium consisting of MS inorganic salts and B 5 Composed of organic ingredients, hormone combinations of different proportions are added, 3% sucrose and 0.8% agar are added, and the pH value is 5.8.

[0029] Callus induction medium and callus subculture medium were added with different hormone combinations (see Table 1). The hormone combination added to the green seedling differentiation medium is: SG 1 , additional CPPU 2.0mg / L, NAA 0.01mg / L; SG 2 , additional KT 0.5mg / L, IAA 0.5mg / L. The strong root...

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Abstract

A technique for high-frequency regrowth of grassiness by somatocyte culture features that the granular embryonic calli are used for culture, the improved culture medium MS is used as basic one, the inducing culture medium contains additionally 2.4-D and KT, the secondary culture medium contains additionally 2,4-D and 6-BA, and the differential culture medium contains additionally CPPU, NAA or KT, and IAA.

Description

1. Technical field [0001] The invention relates to a technical method for obtaining high-frequency plant regeneration through somatic cell culture of Elephant grass, belongs to the field of biotechnology, is specially used for somatic cell culture of Elephant grass, micropropagation of test-tube seedlings and screening of somatic cell mutants, and is suitable for gene transformation mediated by Agrobacterium. 2. Technical Background [0002] Elephant grass (Pennisetum Purpureum Schumach) is a perennial herbaceous plant of the Gramineae Pennisetum genus. It is native to tropical Africa and is a high-yield forage grass suitable for planting in tropical and subtropical regions. It can be used for herbivorous livestock and poultry breeding, road slope protection, medium and high-grade pulp and artificial For wood production, research in recent years has shown that elephant grass is an ideal biomass energy crop, and the planting area at home and abroad has a tendency to expand rap...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00C12N5/04
Inventor 钟小仙佘建明顾洪如梁流芳张建丽丁成龙张保龙倪万潮
Owner JIANGSU ACAD OF AGRI SCI