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Big Symphyllia mucilage collection method

A collection method, coral technology, applied in animal husbandry, etc., can solve problems such as inability to survive, inability to collect mucus, damage to stony corals, etc., and achieve the effect of easy extraction

Inactive Publication Date: 2009-04-08
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although mucus secretion can be enhanced by environmental stresses such as sediment, fresh water, air exposure, etc., it is not sufficient for certain studies that require large amounts of mucus
Although the method of low temperature and adding chemical reagents adopted by the above-mentioned Meikle et al. obtains a large amount of mucus, the low temperature and chemical reagents cause damage to stony corals (which have been listed as protected animals), making the mucus unable to survive after collection; moreover, chemical reagents can affect subsequent Research
[0005] For S. gigantea, it is of great significance to study the compounds in its mucus in terms of chemical ecology and application, but referring to the previous method (without adding chemical reagents), it is impossible to collect a large amount of mucus

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Pry a small piece of huge zygophyllia coral from the coastal reef under the jurisdiction of the Hainan Tropical Marine Biology Experimental Station of the Chinese Academy of Sciences (Luhuitou, Sanya, Hainan), with a surface area of ​​240cm 2 (cover the reef block surface with Oxford paper, then measure the paper area, then draw the surface area of ​​the coral block). Move the pried coral block to the shore, and knock it twice with an external force of 3kg (each interval is less than 10 seconds). Put the coral into a plastic tank filled with seawater, soak for 60 minutes, take out the coral, put it upside down in a plastic basin passivated with 0.02% Tween 20, and collect the mucus. The amount of mucus collected reached 65ml.

Embodiment 2

[0023] Pry a small piece of huge zygophyte coral from the coastal reef of the Hainan Tropical Marine Biology Experimental Station of the Chinese Academy of Sciences (Luhuitou, Sanya, Hainan), with a surface area of ​​320cm 2 (cover the reef block surface with Oxford paper, then measure the paper area, then draw the surface area of ​​the coral block). Move the pried coral block to the shore, and knock it three times with an external force of 2.5kg on the top (with an interval of less than 10 seconds each time). Put the coral into a plastic tank filled with seawater, soak for 70 minutes, take out the coral, put it upside down in a plastic basin passivated with 0.02% Tween 20, and collect the mucus. The amount of mucus collected reached 100ml.

Embodiment 3

[0025] Pry a small piece of huge zygophyllum coral from the coastal reef under the jurisdiction of the Hainan Tropical Marine Biology Experimental Station of the Chinese Academy of Sciences (Luhuitou, Sanya, Hainan), with a surface area of ​​330cm 2 (cover the reef block surface with Oxford paper, then measure the paper area, then draw the surface area of ​​the coral block). Move the pried coral block to the shore, and knock it twice with an external force of 3.5kg on the top (with an interval of less than 10 seconds each time). Put the coral into a plastic tank filled with seawater, soak for 80 minutes, take out the coral, put it upside down in a plastic basin passivated with 0.02% Tween 20 in advance, and collect the mucus. The amount of mucus collected reached 110ml.

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PUM

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Abstract

The invention provides a method for collecting mucous from giant S.gigantea, which comprises the following steps: (1) prying out a block of healthy giant S. gigantean of moderate size from seabed; (2) knocking the top of pried out coral with extra force for more than twice; (3) immersing in sea for 50-100 minutes; (4) taking out the coral and putting into collecting basin to collect the mucous; (5) pouring the mucous into plastic centrifuge tube, maintaining under temperature of minus 20 Deg. C. The invention collects the mucous from giant S. gigantean by means of physical stimulation, which avoids great damage to coral and guarantee the survival of coral after returning to sea, and the nonuse of chemical agent makes little influence to further research.

Description

technical field [0001] The present invention relates to a method of collecting the mucus of the giant phyllophyllum for use and research. Background technique [0002] Coral reef ecosystems are among the most productive and diverse systems on Earth, however, in recent decades many scientists have invested in the disease resistance of stony corals as disease outbreaks have killed coral polyps and led to reef bleaching. researching. The mucus secreted by coral polyps covers the surface of the reefs formed by coral polyps. This layer of mucus is the first gateway for corals to resist pathogens. Therefore, it is very important to study the anti-bacteria effects of mucus. The compounds contained in the mucus can not only serve as anti-infection substances of the coral itself, but also the biological activity of many compounds can also provide humans with antibiotics, anti-tumor agents and other drugs. [0003] Although the study of mucus in stony corals is of great significance...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/00
Inventor 陈国华黄良民谭烨辉
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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