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Quick method for detecting whether blended with remade milk in fresh milk

An inspection method and technology for fresh milk, applied in the preparation of test samples, measurement of color/spectral characteristics, etc., can solve the problems of complex processing process, long time consumption, long time, etc., and achieve high sensitivity, simple use, and easy to grasp. effect of operation

Inactive Publication Date: 2009-04-08
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no domestic method for the adulteration of reconstituted milk in fresh milk; foreign countries have: 1), the measurement method of visible spectrum and ultraviolet spectrum (700-240nm), but the method has complicated processing steps and takes a long time. long; 2), use HPLC to detect furosine to detect whether reconstituted milk is mixed in raw milk and pasteurized milk, but the standard required by this method is expensive and the processing steps are complicated; 3), measure the δD and δD of water in milk 18 O stable isotope ratio to identify whether fresh milk is mixed with reconstituted milk, but this method has the disadvantages of complicated process, long time-consuming and high cost
For various reasons, the above methods cannot be widely used.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1. A quick test method for whether fresh milk is mixed with reconstituted milk, the following steps are carried out in sequence:

[0024] 1) Take 1ml of the milk sample to be tested in a centrifuge tube with a stopper; then add 6ml of acetic acid and sodium acetate buffer solution with a pH of 4.6 and 0.1mol / L to the centrifuge tube with a stopper, mix well and let stand for 5 minutes , and then centrifuged at 3500g to 4500g for 8 to 12 minutes at a temperature of 10 to 20°C to obtain a supernatant;

[0025] 2), the above supernatant is filtered with a 0.45 μm water-based filter membrane to obtain a filtrate;

[0026] 3), select the above-mentioned 0.1ml filtrate to mix with 5ml water to make the solution to be tested;

[0027] 4), take above-mentioned liquid to be tested, take the bovine serum albumin of 1mg / L as external standard, measure on the fluorescence spectrophotometer; The fluorescence value of the Rad reaction product; when adjusting the excitati...

Embodiment 2

[0029] Embodiment 2, a kind of rapid inspection method whether reconstituted milk is mixed in fresh milk, carries out following steps successively:

[0030] 1) Take 1ml of the milk sample to be tested in a centrifuge tube with a stopper; then add 5ml of acetic acid and sodium acetate buffer solution with a pH of 4.6 and 0.1mol / L to the centrifuge tube with a stopper, mix well, and let it stand for 4~ 6 minutes, and then centrifuged at 3500g-4500g at a temperature of 10-20°C for 8-12 minutes to obtain a supernatant;

[0031] 2), the above supernatant is filtered with a 0.45 μm water-based filter membrane to obtain a filtrate;

[0032] 3), select the above-mentioned 0.1ml filtrate and 4ml water to mix to make the solution to be tested;

[0033] 4), take above-mentioned liquid to be tested, take the bovine serum albumin of 1mg / L as external standard, measure on the fluorescence spectrophotometer; The fluorescence value of the Rad reaction product; when adjusting the excitation ...

Embodiment 3

[0035] Embodiment 3, a kind of rapid inspection method whether reconstituted milk is mixed in fresh milk, carries out following steps successively:

[0036] 1) Take 1ml of the milk sample to be tested in a centrifuge tube with a stopper; then add 7ml of acetic acid and sodium acetate buffer solution with a pH of 4.6 and 0.1mol / L to the centrifuge tube with a stopper, mix well, and let it stand for 4~ 6 minutes, and then centrifuged at 3500g-4500g at a temperature of 10-20°C for 8-12 minutes to obtain a supernatant;

[0037] 2), the above supernatant is filtered with a 0.45 μm water-based filter membrane to obtain a filtrate;

[0038] 3), select the above-mentioned 0.1ml filtrate to mix with 6ml water to make the solution to be tested;

[0039]4), take above-mentioned liquid to be tested, take the bovine serum albumin of 1mg / L as external standard, measure on the fluorescence spectrophotometer; The fluorescence value of the Rad reaction product; when adjusting the excitation ...

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PUM

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Abstract

The method of quickly detecting blended reduced milk in fresh milk includes the following steps: 1. mixing milk sample to be detected, acetic acid and sodium acetate buffering liquid and centrifuging to obtain supernatant; 2. filtering the supernatant to obtain filtrate; 3. mixing the filtrate and water to obtain the liquid to be measured; 4. measuring the liquid to be measured in spectrofluorophotometer with 1 mg / L ox serum albumin as external standard to obtain fluorescence value of Mailard reaction product and fluorescence value of tryptophane; and 5. dividing the fluorescence value of Mailard reaction product with the fluorescence value of tryptophane and multiplying by 100 to obtain the test value. The raw milk with test value smaller than 10 is qualified product without reduced milk, and the pasteurized milk with test value smaller than 11.5 is qualified product without reduced milk. The said method is fast, simple and sensitive.

Description

technical field [0001] The invention relates to a quick test method for whether reconstituted milk is mixed in fresh milk. Background technique [0002] With the improvement of people's living standards, the consumption of dairy products in China has increased rapidly; accelerating the development of the dairy industry and improving the consumption level of residents' milk products are the food development areas prioritized in the "Outline of China's Food and Nutrition Development (2001-2010)" . In recent years, the growth rate of dairy industry consumption of urban residents in my country has been above 20%. In 2003, the per capita milk consumption (fresh milk) of urban residents has reached about 25 kg. Drinking liquid milk such as pasteurized milk has gradually become a popular trend in my country. It has become a habit, and liquid milk has also become the main dairy product in my country. [0003] Among the main products of liquid milk, pasteurized milk is the main milk...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/33G01N1/28
Inventor 关荣发叶兴乾刘东红童军锋陈健初
Owner ZHEJIANG UNIV