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Composite microbial preparation for treating solid waste and its preparation process

A compound bacterial agent and organic solid technology, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of shortening the treatment time of organic solid waste, and achieve the effect of improving decomposition rate and accelerating degradation

Inactive Publication Date: 2009-05-13
北京合百意生态能源科技开发有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The object of the present invention is to provide a kind of composite bacterial agent for treating organic solid waste and its preparation method, this composite bacterial agent fundamentally solves the defect that the mixed flora existing in the prior art cannot exert their respective advantages, and its Bacteria that are cultivated separately and mixed together are reasonably matched and have a wide range of applications. They can give full play to the biological characteristics and mutual coordination effects of each flora, significantly shorten the treatment time of organic solid waste, improve the utilization rate of raw materials and the treatment effect and The yield and quality of the product, the strains in the artificial culture process are stable, the preparation method is simple, the growth rate of the flora is fast, and it is beneficial to the treatment of organic solid waste without any environmental pollution

Method used

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Examples

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Effect test

preparation example Construction

[0025] The preparation method of the composite bacteria agent for treating organic solid wastes is that each bacteria is respectively cultured, dried, and finally prepared and mixed according to the proportion, and then packaged. The weight ratio of each bacteria in the composite bacteria agent is 9-13 parts of Q-0 bacteria, 2-4 parts of Q-3 bacteria, 3-4 parts of Tri-1 bacteria, and 2-3 parts of L-1 bacteria.

[0026] The individual cultivation operation steps of described each bacterium are as follows:

[0027] (1) Preparation of Thermomonas CGMCC1364

[0028] Put the above-mentioned preserved thermomonads back on the sterilized culture medium, cultivate them from the slope to the culture tank, adopt the step-by-step expansion culture method, and cultivate them at a culture temperature of 45-60 °C, and produce them in the culture tank After culturing for 24 to 48 hours, the culture solution was obtained, mixed with sterilized solid medium, culture solution: solid medium = 1...

Embodiment 1

[0035] Example 1 Preparation of a composite bacterial agent for treating organic solid waste

[0036] First, each bacteria is cultured and dried separately, and finally mixed according to the proportion and then packaged. The weight ratio of each bacteria in the composite bacterial agent is 9 parts of Q-0 bacteria, 4 parts of Q-3 bacteria, 4 parts of Tri-1 bacteria, and 3 parts of L-1 bacteria.

[0037] The individual cultivation operation steps of described each bacterium are as follows:

[0038] 1. Preparation of Tri-1 bacteria

[0039] (1) First prepare the test tube inclined plane

[0040] The medium adopts PDA medium, and its components are: 200 g of potatoes peeled and cut into small pieces, and 20 g of sucrose. Boil the potatoes and filter, take the filtrate and add water to 1000ml, add sucrose, add 15g of agar to dissolve, put it into a test tube, and sterilize it to make a slope. The original seed was inoculated on the slant, and the spores grew after 120 hours in a...

Embodiment 2

[0073] Example 2 Preparation of a composite bacterial agent for treating organic solid waste

[0074] First, each bacteria is cultured and dried separately, and finally mixed according to the proportion and then packaged. The weight ratio of each bacteria in the composite bacterial agent is 10 parts of Q-0 bacteria, 3 parts of Q-3 bacteria, 3 parts of Tri-1 bacteria, and 2.5 parts of L-1 bacteria.

[0075] The individual cultivation operation steps of described each bacterium are as follows:

[0076] 1. Preparation of Tri-1 bacteria

[0077] (1) First prepare the test tube inclined plane

[0078] The medium adopts PDA medium, and its components are: 200 g of potatoes peeled and cut into small pieces, and 20 g of sucrose. Boil the potatoes and filter, take the filtrate and add water to 1000ml, add sucrose, add 15g of agar to dissolve, put it into a test tube, and sterilize it to make a slope. Inoculate the original seed on the slant, put it in a 30°C incubator and cultivate...

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Abstract

The present invention relates to one kind of composite microbial preparation for treating solid waste and its preparation process. The composite microbial preparation is compounded with CGMCC No. 1364 high temperature monad, CGMCC No. 1365 Bacillus subtilis, CGMCC No.1366 trichoderma and CGMCC No. 1.813 Buccillus licheniformis. It is superior to available composite microbial preparation, and has the advantages reasonable microbe variety matching, wide application range, obvious synergistic effect, short solid waste treating period, no environmental pollution, etc.

Description

technical field [0001] The invention relates to a microbial compound bacterial agent and a preparation method thereof, in particular to a compound bacterial agent for biological treatment of organic solid waste, such as crop straw, solid livestock and poultry manure, urban organic waste, and the like. its preparation method. The composite bacterial agent can also be used for biological treatment of sewage or waste water. Background technique [0002] As we all know, the biological treatment of organic solid waste applied to biogas fermentation is a very effective technology for controlling the pollution of organic solid waste to the environment and developing renewable energy. In the process of practical application, it was found that in the organic solid waste treated by the biogas fermentation process, especially in various crop straws and urban organic waste, it contains refractory cellulose and lignin, which are decomposed by the microbial flora in the natural environme...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/00C12N1/14C12N1/20C12R1/125C12R1/885C12R1/10
Inventor 刘晓风颜开廖银章张海涛袁月祥颜丽刘克鑫
Owner 北京合百意生态能源科技开发有限公司
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