Supercharge Your Innovation With Domain-Expert AI Agents!

Plasmid having three complete transcriptional units and immunogenic compositions for inducing an immune response to HIV

A technology of immunogenicity and composition, which is applied in the field of plasmids and immunogenic compositions to improve the immune response of preventive and therapeutic antigens, and can solve the problems of carrier instability and the possibility of increasing repetitive sequence recombination

Active Publication Date: 2007-07-18
WYETH LLC
View PDF60 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The presence of homologous sequences within the plasmid would make the plasmid unsuitable for use in DNA immunogenic compositions because the presence of homologous sequences in the plasmid backbone increases the likelihood of recombination between repeat sequences and leads to vector instability

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Plasmid having three complete transcriptional units and immunogenic compositions for inducing an immune response to HIV
  • Plasmid having three complete transcriptional units and immunogenic compositions for inducing an immune response to HIV
  • Plasmid having three complete transcriptional units and immunogenic compositions for inducing an immune response to HIV

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0241] Example 1. Selection and modification of HIV genes

[0242]Those skilled in the art will appreciate that sequence information from many viruses and bacteria is available in the art. More specifically, the sequence information can be used to clone the genes used to express the polypeptides in the plasmids of the invention. Information on many sequences from HIV and other pathogens is available from the Los Alamos National Laboratory's HIV Sequence Database and the United States National Library of Medicine's National Biotechnology Available from the National Center for Biotechnology Information (8600 Rockville Pike, Bethesda, MD 20894).

[0243] In one embodiment of the invention, the following HIV genes were selected for inclusion in an exemplary single DNA plasmid expressing a large portion of the HIV genome: the gag gene from the HXB2 isolate and the pol gene from the HXB2 isolate. The complete HXB2 sequence is listed in the GenBank computer database under accession...

example 2

[0254] Example 2. Construction of single, double and triple transcription unit plasmids

[0255] The plasmids detailed in these examples are listed in Tables 1 and 2.

[0256] Construction of triplet transcription unit expression cassettes in circular double-stranded DNA plasmids with the aid of multiple components. See Figure 1. The first component is a first transcription unit for expression of the polypeptide in eukaryotic cells, which consists of the simian cytomegalovirus (SCMV) promoter, a cloning site and bovine growth hormone (BGH) poly-A signal. The second component is a second transcription unit for expressing polypeptides in eukaryotic cells, which consists of the human cytomegalovirus (HCMV) immediate early promoter, a cloning site and SV40 polyadenylation (poly A) signal . Separating the first and second transcription units is a spacer 1 . The third component is a third transcription unit for expression of polypeptides in eukaryotic cells and consists of the h...

example 3

[0257] Example 3. Triple transcription unit plasmid containing six HIV genes

[0258] As an illustration of the use of triple transcription unit plasmid DNA vectors, vectors capable of co-expression of three eukaryotic open reading frame plasmids were formed. The triple transcription unit plasmid DNA vector was formed by inserting the following selected genes encoding HIV-1 antigens into the triple transcription unit expression cassette described in Example 2. All cloning techniques were performed according to conventional techniques (Sambrook et al., 1989).

[0259] First, an HIV-1 gag-pol fusion gene is inserted into the Pmel-Xhol cloning site between the SCMV site of the first transcription unit and the BGH poly-A site. The gag gene was derived from a HXB2 isolate, and similarly, the pol gene was also derived from a HXB2 isolate. The complete HXB2 sequence is listed in the GenBank computer database under accession number K03455. Those skilled in the art will appreciate t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a DNA plasmid comprising: (a) a first transcriptional unit comprising a nucleotide sequence that encodes a first polypeptide operably linked to regulatory elements including a first promoter and a first polyadenylation signal; (b) a second transcriptional unit comprising a nucleotide sequence that encodes a second polypeptide operably linked to regulatory elements including a second promoter and a second polyadenylation signal; (c) a third transcriptional unit comprising a nucleotide sequence that encodes a third polypeptide operably linked to regulatory elements including a third promoter and a third polyadenylation signal; and wherein said first, said second and said third promoters are each derived from different transcriptional units; and wherein said first, said second and said third polyadenylation signals are each derived from different transcriptional units. The invention further relates to immunogenic compositions for inducing an immune response to HIV comprising combinations of two, three, or four plasmids, where each plasmid is expressing a defined antigen, which may be a single antigen or a fusion of two or three antigens.

Description

technical field [0001] The present invention relates to plasmids, immunogenic compositions and methods for improving the immune response to prophylactic and therapeutic antigens. Background technique [0002] Immunization with DNA plasmid-based immunogenic compositions is a powerful tool that can be used to develop methods for the prevention or treatment of infectious diseases or for the treatment of ongoing disease processes. Plasmid DNA immunization has been extensively tested in animal models where it has been found to be effective in inducing cellular and humoral immune responses against a variety of infectious agents and tumor antigens. See Donnelly JJ et al., Ann. Rev. Immunol.; 15:617-48 (1997); Iwasaki A et al., J Immunol 158(10):4591-601 (1997); Wayne, C.L. and Bennett M., Crit . Rev. Immunol., 18: 449-484 (1998). [0003] An important advantage of plasmid DNA immunization is that it allows genes to be cloned, modified, and placed into potential plasmid DNA expres...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/63
Inventor 马尼恩德尔·K·锡德伍约翰·H·埃尔德里奇迈克尔·伊根齐姆拉·伊斯雷尔
Owner WYETH LLC
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com