Artificial synthetic Bt antiinsect gene used for transgene antiinsect plant and its development method

A gene and insecticidal protein technology, applied in the field of Bacillus thuringiensis Btcry gene and its development, can solve problems such as unclear sequence
CN1513868AInactive Publication Date: 2004-07-21INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
1 Cites 11 Cited by

Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
Publication Date
2004-07-21
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

An artificially synthetic insecticiding gene Bt used for insect-resisting transgenic plant is prepared from the high-toxic Bt CryIIe protein of lepidopterous pest through insecticiding activity deficiency, amplifying the termination-deficient gene fragment, configuring expression carrier, transforming it to obtain recombinant engineered bacteria, inducing expression analyzing insecticiding activity, determining the minimal terminal active region and active region, reinforming polyadenylation signal sequence and ATTTA sequence, optimiizng plant prefaverable codon, and artificial synthesis.
Need to check novelty before this filing date? Find Prior Art

Description

Technical field:

[0001] The invention belongs to the technical field of biological control. Specifically, it relates to the Btcry gene of Bacillus thuringiensis for plant expression and its development method. Background technique:

[0002] Bacillus thuringiensis (Bt for short) is a Gram-positive bacterium with an extremely wide distribution. While forming spores, it can produce protein-like parasporal crystals (parasporal crystal), for Lepidoptera (Lepidoptera), Diptera (Diptera), Coleoptera (Coleoptera), Hymenoptera (Hymenoptera), Homoptera (Homoptera), Orthoptera (Orthoptera), Mallophaga (Mallophaga) and other insects, as well as nematodes, mites and protozoa have specific insecticidal activity (Schnepf.E., N. etc., Microbiol.And Mocecular Biology Review, 1998, 62: 3 775-806). Insecticidal Crystal Proteins (ICPs), also known as delta-endotoxin (delta-endotoxin), are harmless to humans and animals, and do not pollute the environment. Therefore, Bt is the most widely used in the...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More