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Seawater domestication and culture method of daphniopsis tebitana sars

A technology of domestication and cultivation of seawater, which is applied in the field of seawater domestication and cultivation of Daphnia fructus in Tibet, so as to improve the survival rate of fry and reduce the cost of raising seedlings

Inactive Publication Date: 2011-06-08
DALIAN FISHERIES UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But so far, there has not been a large amount of artificial cultivation and utilization of Daphnia tibetan with seawater (salinity 30-35) in the northern seawater fish breeding season (April-June).

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] a. Disinfect and sterilize the culture container by conventional methods, and the surroundings of the culture container are opaque;

[0027] b. Deploying water for cultivation: detoxify and filter seawater, adjust the pH of seawater to 7-8 with acid and alkali, use electric heating or light to make the temperature 10-15°C, and adjust the salinity to 10-15 with fresh water;

[0028] c. Select species of daphnia: put the collected daphnia tibetan in the culture container described in step a, and cultivate it with the culture water described in step b. Feed the bait such as chaetoceros moowii, and the amount of feeding is 3×10 5 cell / ml, after 1-2 litters of juvenile daphnia have been born, healthy female daphnia with a body length of 2-3 mm and active swimming are selected as seed daphnia;

[0029] d. To cultivate:

[0030] Use the culture container described in step a, and inject the algae liquid of 1 / 5 to 1 / 4 of the volume of the culture container. Produced by one or...

Embodiment 2

[0037]The basic culture method is the same as in Example 1, the culture container of the a step is a 20-251 glass jar or a plastic water tank, soaked in a 10% HCl solution for at least 6 hours, then rinsed with fresh water, the glass jar or plastic water tank Cover it with opaque cloth or paper around it.

Embodiment 3

[0039] The basic cultivation method is the same as that in Example 1 or Example 2. The salinity in step b is obtained by blending nearshore seawater through sand filtration, sedimentation, and 20 μm sieve filtration with tap water after aeration, and aerating for 3 to 5 hours after deployment. . pH can use NaHCO 3 Adjust the pH of diluted seawater with a salinity of 10-15 to 7-8 and the alkalinity to 7-12mmol / l with HCl. It is best to add 0.6-1.0g of NaHCO per liter of water 3 , and then adjust the pH to 7-8 with HCl.

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Abstract

The invention discloses a seawater domestication method for Daphniopsis tibetana Sars, which comprise the following steps: disinfecting the culture container, culture container being opaque in periphery; concocting culture liquor, taking disinfected and filtered seawater to adjust pH value to 7-8, temperature 10-15 degree, salinity 10-15, selecting Daphniopsis tibetana Sars and placing it in container, culturing it with above-mentioned liquor, illumination intensity 1000-2000Lx, photoperiod 14D:10L, feeding with Chaetoceros sp. feedstuff, feeding volume 30x105cell / ml, selecting healty, activefemale Daphniopsis tibetana Sars which has produced baby Daphniopsis tibetana Sars and is 2-3 mm in body length, feeding it with algae liquid selecting one or two kinds from Chaetoceros sp., Dunaliella salina, Isochrysis galbana, chlorella, feeding density 30x105cell / ml, inoculating Daphniopsis tibetana Sars density 100ind / l, observing Daphniopsis tibetana Sars growth state and water body to partly for wholly substitute for Artemia and promote fish fry survival rate, reduce the seedling-breeding cost.

Description

Technical field: [0001] The invention relates to a method for acclimating and cultivating Tibetan Daphnia in seawater. Background technique: [0002] Daphniapsis tibetana Sars, 1903, belongs to the genus Daphniapsis of the family Daphniidae, and is mainly distributed in my country's Tibet, Qinghai, Xinjiang and other places, and abroad in the former Soviet Union, India, Pamirs (syn.Daphniapamiresis Rylov), Iran (syn.Daphnia fusca Gurney) and Antarctica (syn.Daphnia studerri Rühe) are a kind of cold-water saline cladocera. The natural water temperature of its distribution is -2~18℃, salinity 9~35, pH9~10.4 . Because Daphnia tibetan likes low temperature and tolerates high salinity, it is suitable for living in high-altitude, high-cold, and nutrient-poor water bodies. It can become a new live bait in the cultivation of fish, shrimp and crab in northern sea water, especially for the relay production of marine fish seedlings. After rotifers are used as opening bait, a large numb...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K61/00A61L2/18
CPCY02A40/81
Inventor 赵文霍元子
Owner DALIAN FISHERIES UNIVERSITY
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