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New campylobacterium LF-3 and its application

A technology of Bacillus flexus, LF-3, applied in the field of phytotoxin biodegradation, can solve the problem of TMPD toxicity reduction

Inactive Publication Date: 2007-11-07
NORTHWEST A & F UNIV
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Currently, there is no effective way to reduce the toxicity of TMPD in Oxytropis glacialis

Method used

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  • New campylobacterium LF-3 and its application
  • New campylobacterium LF-3 and its application
  • New campylobacterium LF-3 and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment 1: the separation and screening method of Bacillus flexus LF-3

[0036] 1. Soil samples: collected from the environmental soil where locoweeds grow in Tibet, Inner Mongolia, Qinghai and other places;

[0037] 2. Isolation and screening of bacterial strains: Take 20g~30g of soil from 4 sources in the growth environment of locoweed and inoculate them in ordinary broth, expand the bacteria at 37°C for 48 hours, then inoculate them on ordinary agar by streaking, culture at 37°C for 24 hours, Pick a single colony and inoculate it in common broth containing 50mg / L TMPD, incubate at 37°C for 48 hours, and save the strain; inoculate the strain saved in the previous step in common broth containing 100mg / L TMPD, place at 37°C Cultivate for 48 hours; repeat the above process, increase the content of TMPD each time, reduce the content of organic nutrients in the culture solution, and preserve the strains. After 5 times of cultivation, when the content of TMPD in the med...

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Abstract

The present invention discloses one kind of new Bacillus flexus LF-3 in the preservation number of CCTCC M 206128. The Bacillus flexus LF-3 can degrades phytotoxin effectively, and eliminate toxicity of the alkaloid 2, 2, 6, 6-tetramethyl piperidone (TMPD) extracted and separated from the above-ground parts of Oxytropis glacialis as one kind of poisonous grassland weed. The present invention is significant in eliminating the poisoning of Oxytropis glacialis and the biological control and ecological research of Oxytropis glacialis.

Description

technical field [0001] The invention belongs to the technical field of biodegradation of plant toxins, in particular to TMPD-degrading bacteria, and also relates to the application of the TMPD-degrading bacteria in degrading TMPD, the main toxin of Oxytropis glacier. Background technique [0002] Locoweed is the general term for the poisonous plants of the genus Astragalus and Oxytropis in the leguminous family, and it is also the most serious poisonous weed that endangers grassland animal husbandry in the world. In my country, locoweeds are widely distributed in the vast grasslands of Northwest, North and Southwest China, with an area of ​​damage reaching 11 million ha 2 . In recent years, important progress has been made in domestic and foreign research on the hazards of locoweeds, toxic components, toxicological mechanisms, prevention and utilization, but so far there is no effective method to control the spread of locoweeds, which has caused the problem of locoweed pois...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/74C12R1/07A61K35/742
Inventor 王建华李勤凡王岚峰耿果霞
Owner NORTHWEST A & F UNIV
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