Method for preparing A-group streptococcus agent by bloodless medium and bloodless medium

A blood-free culture medium, the technology of group A streptococcus, which is applied to the preparation of group A streptococcus preparations with blood-free culture medium, and the field of blood-free culture medium, can solve the problems of increasing the content of pyrogen substances, allergic reactions of patients, and high cost, and achieves the The effect of reducing xenogeneic substances, simplifying experimental procedures, and reducing blood components

Inactive Publication Date: 2008-01-02
CHANGCHUN UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the common use of blood and plasma media in the traditional preparation process, the cost of the preparation is relatively high, and it is easy

Method used

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  • Method for preparing A-group streptococcus agent by bloodless medium and bloodless medium

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] 1.0% tryptone, 0.8% glucose, 0.2% yeast powder, 0.5% sodium chloride, 0.5% potassium dihydrogen phosphate, and the rest are water; the pH value is 7.4 to prepare the medium, and the biological yield is 0.2055g according to the regression equation / l. In the actual test, the biological yield of 0.1997g / l was obtained, and the biological yield of the blood medium containing the same ratio was 0.2010g / l.

Embodiment 2

[0043] According to 0.8% of tryptone, 0.7% of glucose, 0.3% of yeast powder, 0.5% of sodium chloride, 0.5% of potassium dihydrogen phosphate, and the rest are water; the pH value is 7.5 to prepare the culture medium, and the biological yield is calculated according to the regression equation to be 0.3240g / l. In the actual test, the biological yield of 0.3302g / l was obtained, and the biological yield of the blood medium containing the same proportion was 0.3260g / l.

Embodiment 3

[0045] 1.2% of tryptone, 0.9% of glucose, 0.4% of yeast powder, 0.5% of sodium chloride, 0.5% of potassium dihydrogen phosphate, and the rest are water; the pH value is 7.6 to prepare the medium, and the biological yield is 0.2389g according to the regression equation / l. In the actual test, the biological yield of 0.2410g / l was obtained. The biological yield of the blood medium containing the same proportion was 0.2410g / l.

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Abstract

The invention discloses a preparing method of A-group streptococcus agent and avascular culture medium, which comprises the following steps: generating A-group streptococcus weak-toxic strain working seed; preparing seed liquid in the avascular culture medium; inactivating the cultured material; freezing and drying; obtaining the product. The avascular culture medium contains 1.2% tryptone, 0.9% glucose, 0.4% yeast powder, 0.5% sodium chloride, 0.5% potassium dihydrogen phosphate and water, wherein the pH value is 7.4-7.6.

Description

technical field [0001] The invention belongs to the field of microbial fermentation engineering, and relates to a method for preparing a group A streptococcus preparation by using a blood-free medium instead of a blood medium, in particular to a method for preparing a group A streptococcus preparation by using a blood-free medium and bloodless culture base. Background technique [0002] Group A Streptococcus preparations, also known as OK-432, are biotherapeutic drugs. They are freeze-dried products made of attenuated strains of Group A Streptococcus after cultivation, amplification, and penicillin treatment. They have been used in clinical treatment, such as cancerous pleural effusion And immunotherapy of malignant tumors, etc., its performance is stable and its anticancer effect is remarkable. However, due to the common use of blood and plasma media in the traditional preparation process, the cost of the preparation is relatively high, and it is easy to bring in heterolog...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/46
Inventor 邱芳萍吴战宇卢日峰郎维
Owner CHANGCHUN UNIV OF TECH
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