Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for preparing A-group streptococcus agent by bloodless medium and bloodless medium

A blood-free culture medium, the technology of group A streptococcus, which is applied to the preparation of group A streptococcus preparations with blood-free culture medium, and the field of blood-free culture medium, can solve the problems of increasing the content of pyrogen substances, allergic reactions of patients, and high cost, and achieves the The effect of reducing xenogeneic substances, simplifying experimental procedures, and reducing blood components

Inactive Publication Date: 2008-01-02
CHANGCHUN UNIV OF TECH
View PDF0 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the common use of blood and plasma media in the traditional preparation process, the cost of the preparation is relatively high, and it is easy to bring in heterologous substances, increase the content of pyrogen substances, and injection administration may easily cause allergic reactions in patients.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for preparing A-group streptococcus agent by bloodless medium and bloodless medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] 1.0% tryptone, 0.8% glucose, 0.2% yeast powder, 0.5% sodium chloride, 0.5% potassium dihydrogen phosphate, and the rest are water; the pH value is 7.4 to prepare the medium, and the biological yield is 0.2055g according to the regression equation / l. In the actual test, the biological yield of 0.1997g / l was obtained, and the biological yield of the blood medium containing the same ratio was 0.2010g / l.

Embodiment 2

[0043] According to 0.8% of tryptone, 0.7% of glucose, 0.3% of yeast powder, 0.5% of sodium chloride, 0.5% of potassium dihydrogen phosphate, and the rest are water; the pH value is 7.5 to prepare the culture medium, and the biological yield is calculated according to the regression equation to be 0.3240g / l. In the actual test, the biological yield of 0.3302g / l was obtained, and the biological yield of the blood medium containing the same proportion was 0.3260g / l.

Embodiment 3

[0045] 1.2% of tryptone, 0.9% of glucose, 0.4% of yeast powder, 0.5% of sodium chloride, 0.5% of potassium dihydrogen phosphate, and the rest are water; the pH value is 7.6 to prepare the medium, and the biological yield is 0.2389g according to the regression equation / l. In the actual test, the biological yield of 0.2410g / l was obtained. The biological yield of the blood medium containing the same proportion was 0.2410g / l.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a preparing method of A-group streptococcus agent and avascular culture medium, which comprises the following steps: generating A-group streptococcus weak-toxic strain working seed; preparing seed liquid in the avascular culture medium; inactivating the cultured material; freezing and drying; obtaining the product. The avascular culture medium contains 1.2% tryptone, 0.9% glucose, 0.4% yeast powder, 0.5% sodium chloride, 0.5% potassium dihydrogen phosphate and water, wherein the pH value is 7.4-7.6.

Description

technical field [0001] The invention belongs to the field of microbial fermentation engineering, and relates to a method for preparing a group A streptococcus preparation by using a blood-free medium instead of a blood medium, in particular to a method for preparing a group A streptococcus preparation by using a blood-free medium and bloodless culture base. Background technique [0002] Group A Streptococcus preparations, also known as OK-432, are biotherapeutic drugs. They are freeze-dried products made of attenuated strains of Group A Streptococcus after cultivation, amplification, and penicillin treatment. They have been used in clinical treatment, such as cancerous pleural effusion And immunotherapy of malignant tumors, etc., its performance is stable and its anticancer effect is remarkable. However, due to the common use of blood and plasma media in the traditional preparation process, the cost of the preparation is relatively high, and it is easy to bring in heterolog...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20C12R1/46
Inventor 邱芳萍吴战宇卢日峰郎维
Owner CHANGCHUN UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com