Method for preparing microcapsule having function of specific connecting with tumor cell

A tumor cell and microcapsule technology, which is applied in the field of preparing layered structure microcapsules with a function of specific binding to tumor cells, can solve the problems of non-targeting, short biological half-life, tumorigenicity and the like, and achieves a simple preparation process. , mild conditions

Inactive Publication Date: 2008-01-09
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although cationic liposome delivery technology can greatly improve the introduction efficiency, it has no targeting and short biological half-life; virus-mediated efficiency is high, but virus preparation is difficult, and there is a problem of inducing host immune response, which has potential tumorigenicity

Method used

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  • Method for preparing microcapsule having function of specific connecting with tumor cell
  • Method for preparing microcapsule having function of specific connecting with tumor cell
  • Method for preparing microcapsule having function of specific connecting with tumor cell

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0027] 1) Add 50mL of sodium carbonate solution with a concentration of 0.33M / L to 50mL of calcium nitrate solution with a concentration of 0.33M / L; after 15 minutes, the generated calcium carbonate (expressed as CaCO 3 ) colloidal particles were collected by centrifugation, washed 3 times with water, and stored; the prepared CaCO 3 The scanning electron micrograph of the particles is shown in Figure 1(a);

[0028] 2) 1 mL of CaCO obtained in step 1) with a solid content of 5% 3 The microparticles were placed in a 2mL centrifuge tube, centrifuged to remove the supernatant, and washed 3 times with water; add 1mL polyallylamine hydrochloride (PAH) / 0.5M NaCl solution, shake the centrifuge tube, and wash 3 times with water after 10 minutes. Removal of excess PAH, resulting in CaCO 3 A layer of PAH (expressed as CaCO 3 -PAH); Then add 1mL polystyrene sodium sulfonate (PSS) / 0.5M NaCl solution, shake the centrifuge tube, after 10 minutes, wash with water 3 times to remove excess P...

example 2

[0032] 1) prepare microcapsules with example 1, but in 2) in step, common PAH is replaced by rhodamine-labeled PAH (Rd-PAH), the fluorescent probe label that obtains (PAH / PSS) 4 / Rd-PAH microcapsules and (PAH / PSS) 4 / Rd-PAH / PEG / FA microcapsules;

[0033] 2) Human fibroblasts, SMMC-7721 hepatoma cells and human fibroblasts+SMMC-7721 hepatoma cells were respectively planted on 96-well polystyrene culture plates at a planting density of 10,000 / well; 100 μL containing 10 % (v / v) calf serum and 90% (v / v) DMEM medium, 5% CO at 37°C 2 Cultured in humid air; after 24h, add (PAH / PSS) to each well 4 / Rd-PAH microcapsules, (PAH / PSS) 4 / Rd-PAH / PEG / FA microcapsules and (PAH / PSS) 4 / Rd-PAH / PEG / FA microcapsules + 2.5mM free folic acid solution, the concentration of microcapsules is 2×10 7 / well; slightly shake the mixed culture solution of cells and microcapsules, absorb the culture solution after 15 minutes, add 1mL PBS buffer solution to wash twice; observe the adhesion of microcapsul...

example 3

[0037] 1) Add polystyrene sodium sulfonate (PSS) to the calcium nitrate aqueous solution with a concentration of 0.005M / L, and add a sodium carbonate aqueous solution with a concentration of 0.005M / L under stirring, and the final concentration of PSS in the mixture is 0.5mg / L mL, reacted for 5min under stirring, and centrifuged to obtain CaCO containing PSS inside 3 Colloidal particles (CaCO 3 (PSS)), the scanning electron microscope photo is shown in Figure 1(b);

[0038] Step 2) to step 4) are the same as in Example 1 to obtain PSS-(PAH / PSS) suspended in water 4 / PAH / PEG / FA polyelectrolyte hollow microcapsules.

[0039] 5) Set 2.0±0.2×10 7 PSS-(PAH / PSS) 4 / PAH / PEG / FA microcapsules mixed with 0.5mL 1mg / mL doxorubicin (DOX) aqueous solution, incubated at room temperature for 12h, to obtain microcapsules embedded with DOX, the concentration of DOX in the microcapsules was 24.8mg / mL ; PSS-(PAH / PSS) embedded with DOX 4 / PAH / PEG / FA microcapsules were co-incubated with human ...

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Abstract

The present invention discloses a preparation method of microcapsule which can be specifically combined with tumor cell. Said preparation method includes the following steps: adopting removable colloidal particles as template, utilizing layer-by-layer self-assembly method to make polyelectrolyte with opposite charges be assembled on the surface of colloidal particles, then utilizing polyethylene glycol and tumor cell identification ligand to make modification, finally making the colloidal particles be dissolved or decomposed so as to obtain the invented polymer hollow microcapsule containing tumor cell identification ligand.

Description

technical field [0001] The invention relates to a method for preparing microcapsules, in particular to a method for preparing layered microcapsules with the function of specifically binding to tumor cells. Background technique [0002] The drug-loading system with targeted delivery function plays a very important role in the treatment of tumors. It can improve the availability of drugs, change pharmacokinetics, reduce the damage of chemotherapy drugs to normal tissues and the body's resistance to chemotherapy drugs sex. A variety of structural materials such as liposomes, vesicles, micelles, microparticles and microspheres, hydrogels, and hollow nanocapsules and microcapsules have been used as drug delivery systems. The targeting of the delivery system can be achieved through physical and chemical methods, biological specific recognition and other methods, including direct introduction, liposome delivery, virus-mediated and so on. Although cationic liposome delivery techno...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/50A61K47/32A61P35/00B01J13/06
Inventor 高长有赵庆贺毛峥伟沈家骢
Owner ZHEJIANG UNIV
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