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Method for preparing L-arabinose

A technology of arabinose and amylase, which is applied in the field of preparation of L-arabinose, can solve the problems of unrefined purification, high production cost, and low production efficiency, and achieve the effects of reducing the difficulty of filtration, reducing the content, and improving production efficiency

Inactive Publication Date: 2010-06-09
李萍
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Chinese invention patent CN1299420 proposes a method for producing L-arabinose by acid hydrolysis. The method mainly describes that plant residues containing L-arabinose are selected as raw materials and degraded by acids such as hydrochloric acid and sulfuric acid to prepare L-arabinose. -Arabinose, its disadvantage is that the whole preparation process simply uses hydrochloric acid or sulfuric acid to degrade plant slag raw materials, and the prepared product is a mixed sugar solution containing L-arabinose, xylose, galactose and glucose, etc., and its raw materials The maximum processing capacity is 500g, and the process does not indicate how to refine and purify to obtain the L-arabinose crystal product
CN1366552 also provides a method for preparing and producing L-arabinose, but only selects biological enzyme preparations such as arabinoside degrading enzyme, pectinase, cellulose hemicellulase, etc. to directly degrade the plant slag containing L-arabinose similar substances to obtain L-arabinose, there is also the problem of high production cost and low production efficiency
[0005] In short, the existing technology has certain problems in terms of economy and production efficiency in terms of large-scale preparation and production of L-arabinose

Method used

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  • Method for preparing L-arabinose

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Effect test

Embodiment 1

[0027] Embodiment 1, prepare L-arabinose with the method of the present invention

[0028] Take by weighing 100kg of corn bran after pulverizing, add water and allocate to 5% corn bran material, add 2kg amylase (amylase is made up of α-amylase and β-amylase that the weight and number ratio is 1: 1), 35 Stir the reaction at ℃ for 12h, and filter by centrifugation. The filter cake is washed 2-3 times with water, and the water consumption for each washing is 200L. Add 2000L 1.5% sulfuric acid solution to the reaction kettle containing the above filter cake, reflux at 100°C for 4 hours, stop the reaction, adjust the pH of the reaction solution to 4.5 with calcium carbonate with a pH value of 7, filter, and use 200L 2% ammonia water Wash the filter cake 2 times. Combine the filtrates, add activated carbon with a mass percentage of 1% of the filtrate, decolorize at 100°C for 45 minutes, and filter with a plate frame. The obtained filtrate is passed through 732 cation exchange res...

Embodiment 2

[0029] Embodiment 2, prepare L-arabinose with the method of the present invention

[0030] Take by weighing 150kg of corn bran after pulverizing, add water and allocate into 4% corn bran material, add 2kg amylase (amylase is made up of α-amylase and β-amylase that the weight and number ratio is 0.5: 1), 45 Stir the reaction at ℃ for 8h, and filter by centrifugation. The filter cake is washed 2-3 times with water, and the water consumption for each washing is 220L. Add 2500L 1% hydrochloric acid solution to the reaction kettle containing the above filter cake, reflux at 110°C for 8 hours, stop the reaction, adjust the pH of the reaction solution to 3.5 with calcium hydroxide with a pH value of 8, filter, and use 250L 2% Wash the filter cake with ammonia water 2 times. Combine the filtrates. Add activated carbon with a mass percentage of 2% of the filtrate, decolorize at 105°C for 60 minutes, and filter with a plate and frame filter. The obtained filtrate is passed through 7...

Embodiment 3

[0031] Embodiment 3, prepare L-arabinose with the method of the present invention

[0032]Take by weighing 100kg of corn bran after pulverizing, add water and allocate to 6% corn bran material, add 3kg amylase (amylase is made up of α-amylase and β-amylase that the weight and number ratio is 1.5:1), 40 Stir the reaction at around ℃ for 12h, and filter by centrifugation. The filter cake is washed 2-3 times with water, and the water consumption for each washing is 200L. Add 2000L 5% acetic acid solution to the reaction kettle containing the above filter cake, reflux at 110°C for 6 hours, stop the reaction, adjust the pH of the reaction solution to 5.5 with sodium hydroxide with a pH value of 8, filter, and use 200L 2% Wash the filter cake with ammonia water 2 times. Combine the filtrates. Add activated carbon with a mass percentage of 1.5% of the filtrate, decolorize at 100°C for 90 minutes, and filter with a plate and frame filter. The obtained filtrate is passed through 73...

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Abstract

Preparation of L-pectin sugar is carried out by: processing corn bran with glycase, degrading the corn bran said above with thin acid of 1%-5%, regulating pH3.5-5.5 and collecting supernatant, desalting and removing impurities the supernatant with ion exchanging resin, inoculating yeast and fermenting, removing hetero-monosaccharides, crystallizing the fermented liquid to obtain final product. Itis chemical process combined with biologic one, with simple procedure and high yield and good quality.

Description

technical field [0001] The invention relates to a method for preparing L-arabinose. Background technique [0002] my country is a big agricultural country, and corn is one of the main food crops in our country. Corn starch processing has become a very important part of my country's corn deep processing industry. The content of L-arabinose in corn bran, a by-product of its processing, is as high as 18%. [0003] L-arabinose is a major component of plant cell walls. Because it can inhibit the activity of sucrose invertase and glucose invertase in the human intestine, restrict the conversion of sucrose and glucose into glycogen and be absorbed by the liver, and is safe and reliable, it has become an extremely effective health food and medicine for people to prevent obesity and diabetes. additives. [0004] Chinese invention patent CN1299420 proposes a method for producing L-arabinose by acid hydrolysis. The method mainly describes that plant residues containing L-arabinose a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/02
Inventor 李萍
Owner 李萍
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